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Keywords {🔍}

smg, mrna, upf, pnrc, nmd, doi, pubmed, google, scholar, fig, decay, cells, reporter, pmc, interaction, article, free, levels, rna, decapping, factors, proteins, degradation, protein, nonsensemediated, knockdown, cell, control, expressing, supplemental, complex, tethered, pathway, yeast, assays, mspnrc, mrnas, lanes, mol, plasmids, cho, data, dcp, xrn, biol, human, shown, twohybrid, mühlemann, mammalian,

Topics {✒️}

26 µg pt7-egfp-c1-hsxrn1 xhoi–apai cut pcdna3-flag-smg7 nonsense-mediated mrna decay—mechanisms c-terminal serine-glutamate rich 3 µg pcmv6-mgadph-myc-flag translation-dependent post-transcriptional regulator β-globin-6xms2bs reporter rna mini-µ ter310 mrna plasmid expressing gal4-ad-empty 5 µg pcdna3-flag-myc-dcp1a 11 µg pcmv-ha-ms2-lacz 26 µg pcdna3-flag-myc-dcp2 nonsense-mediated mrna decay plasmid expressing pcdna3-ha-upf1 nonsense-mediated decay pathway β-globin reporter mrna 10 µg pcmv-smg5-ms2-ha mini-µ mrna levels 11 µg pcmv-ms2-ha-pnrc2 psc-a-amp/kan premature translation-termination codon pcmv6-mgadph-myc-flag β-globin mrna levels replication-dependent histone mrna β-globin ter39 mrna asp-glu-ala-asp nonsense-mediated decay factors mini-μ mrna levels pnrc2–smg5-mediated nmd mode nuclear receptor-interacting proteins pmc beta search smg5–pnrc2-mediated degradation pathway 6 µg pcdna3-ha-upf1r β-globin reporter plasmids respective mini-μ mrna peptide z-scores belonging smg5–smg7-mediated deadenylation functions shotgun lc-ms/ms smg5–smg7-mediated nmd pathway chromogenic substrate x-gal premature translation–termination codons 6 µg pcdna3-ha-egfp colonies expressing β-galactosidase post-transcriptional surveillance pathway 100 ng pcβ-globin-6xms2bs pcdna3-ha-egfp plasmid mini-µ ter310 plasmids encoding smg5-ms2-ha lc-ms/ms analysis designated rnai-mediated knockdowns

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