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Keywords {🔍}

prc, binding, doi, dna, rna, fig, histone, nucleosomes, pubmed, google, scholar, complex, nucleosome, pmc, article, free, activity, supplementary, linker, data, chromatin, gel, methylation, jarid, affinity, mer, polycomb, complexes, cell, buffer, hkme, ezh, bind, reaction, studies, proteins, experiments, shown, cpg, min, recruitment, methylated, mol, vivo, genes, protein, reconstituted, binds, marks, regions,

Topics {✒️}

pre-formed prc2-a488-dsdna complexes pre-formed prc2-a488-rna complexes gc-rich sequences genome-wide8 pre-formed prc2-trinucleosome complexes pre-formed prc2-dsdna complexes nucleosomes vis-a-vis rnas pre-formed prc2-rna complex selective methyl-lysine-binding activities current chip-seq datasets high-affinity dna-binding modules protein-free dna controls protein-free linker regions 12 µm s-[methyl-14c]-adenosylmethionine multi-step chromatography approach c2h2 zinc-finger domains protein-free linker dna hotair-mediated transcriptional repression histone-free linker dna prefers gc-rich dna typically nucleosome-free regions15 single-molecule tirf microscopy pfast-bac1 expression vector cg-rich dna regions8 health grants r37-gm086868 nucleosome core/linker junctions dna-barcoded nucleosome libraries jarid2/jumonji coordinates control pre-existing histone modifications pmc beta search ezh2−eed−suz12−rbbp4−aebp2 pre-formed complexes n-terminal tail high-resolution nucleosome mapping resulting prc2-dna complex alter prc2-nucleosome binding prc2−-ezh1 5-mer complexes nucleosome-interacting proteins regulated robust dna-binding activity excess [γ-32p]-atp polycomb-response elements prc2-mediated epigenetic silencing pediatric high-grade gliomas nucleosome-free regions dissociating prc2-nucleosomes complexes prc2-h3k27me3-trinucleosome complexes protein-free dna prc2-nucleosome interactions provide prc2−jarid2 6-mer complex free dsdna gave prc2−ezh1 5-mer complex

Questions {❓}

• How does PRC2 bind chromatin?
• How does PRC2 bind chromatin?
• How does RNA binding regulate PRC2?
• Nucleosome positioning: how is it established, and why does it matter?
• What defines a gene target for PRC2-mediated epigenetic silencing?
• What in vivo data relate to our findings?
• While the hidden specificity in promiscuous RNA-binding has been revealed, the question of RNA functionality still remains: what role do RNA molecules play in the regulation of PRC2?
• Why is the PRC2 gene-silencing complex recruited to actively transcribed genes?
• Yet, do such interactions provide sufficient binding energy, given measured affinities of histone peptide-PRC2 in the 40–400 µM range?

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• https://www.linkedin.com/company/ncbinlm
• https://twitter.com/nlm_nih
• https://www.facebook.com/nationallibraryofmedicine
• https://www.youtube.com/user/NLMNIH

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