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Title[redir]:
Imaging the beating heart in the mouse using intravital microscopy techniques - PMC
Description:
Real-time microscopic imaging of moving organs at single-cell resolution represents a major challenge in studying complex biology in living systems. Motion of the tissue from the cardiac and respiratory cycles severely limits intravital microscopy ...
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Keywords {🔍}
imaging, heart, cardiac, images, acquisition, image, stabilizer, microscope, microscopy, data, doi, animal, supplementary, intravital, protocol, gating, signal, tissue, ventilator, ecg, cat, mouse, motion, file, open, fig, laser, step, cycle, pacing, pmc, figure, software, care, pubmed, google, scholar, surface, timing, olympus, min, time, anesthesia, parameters, article, steps, acquired, prospective, critical, program,
Topics {✒️}
noise ratio motion-artifact-free motion artifact–free measurement motion artifact–free imaging final artifact-free reconstruction solid-state diode lasers motion artifact–free images �cont acq&graph voltage materials nihms852525-supplement-sup_materials olympus fv1000-mpe system integrating pathway-specific information pmc beta search olympus fv1000-mpe confocal langendorff-perfused mouse hearts low laser-intensity excitation endoscopic time-lapse imaging real-time cardiac gating physiologic motion–induced artifacts real-time 2-photon imaging �-inch insulin syringe custom-made matlab program single-cell resolution represents medical application–approved material 1/8-inch-thick aluminum plate higher-speed acquisition based assisted motion-synchronized acquisition typical laser-scanning confocal demonstrates subcellular-resolution images banana plug connectors open-source software packages custom-designed tissue stabilizer minimize excessive motion bnc terminal–labeled ‘sync higher-power laser illumination general signal-processing algorithm microscope time-frame window sequential segmented microscopy red banana jacks procedure laser-scanning microscope manufacturer-supplied expiration date graphical user interface microscope frame-acquisition frequency respiratory motion artifact custom-made platforms select ‘real-time—8 hz sapphire laser suitable red ‘run/stop’ button femtosecond laser unit cardiac-induced motion adapters’ black jacks primary skin incision
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