
NCBI . NLM . NIH . GOV {
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Title[redir]:
SIRT5 promotes IDH2 desuccinylation and G6PD deglutarylation to enhance cellular antioxidant defense - PMC
Description:
Excess in mitochondrial reactive oxygen species (ROS) is considered as a major cause of cellular oxidative stress. NADPH, the main intracellular reductant, has a key role in keeping glutathione in its reduced form GSH, which scavenges ROS and thus ...
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Keywords {đ}
sirt, idh, cells, activity, cell, gpd, doi, pubmed, google, scholar, oxidative, data, nadph, fig, ros, cellular, lysine, results, pmc, mefs, knockdown, succinylation, protein, experiments, paraquat, free, article, hekt, average, twotailed, unpaired, ttest, enzyme, determined, independent, enzymes, figure, flag, mitochondrial, materials, methods, levels, mice, stress, redox, desuccinylase, production, glutarylation, analysis, assay,
Topics {âď¸}
iceâcold npâ40 buffer quantitative realâtime pcr quantitative rtâpcr showed alphaâketoglutarateâdependent dioxygenases liquid chromatographyâmass spectrometry pmc beta search mitochondriaâspecific antioxidant enzyme singlet oxygenâinduced apoptosis fluor de lysÂŽâsuccinyl nadphâproducing metabolic enzymes largeâscale proteomic surveys major nadphâproducing enzymes mptpâinduced mice model rosâinduced cell death realâtime pcr mitochondrial dysfunctionârelated disease succinylation/malonylation/glutarylation substrates regulated activating nadphâproducing g6pd identified nadphâproducing enzymes expressed flagâtagged protein flagâtagged idh2 proteins h2o2 doseâdependently activated h2o2 doseâdependently activates geneâspecific primers purified flagâtagged idh2 free radical theory mouse embryonic fibroblasts purified flagâtagged g6pd enzymeâindependent nepsilonâacetylation license information pmcid previous proteomic studies maintain nadph homeostasis immunoprecipitated flagâtagged g6pd sirt5âdependent mechanism chemical oxidants enhance nadphâproducing enzyme nadphâproducing enzymes female sirt5 ko desuccinylase activity assay candidate longevity gene regulating nadph homeostasis sirt5 deficiency inhibits lower [nadph/nadp+] ratio oligomycinâsensitive oxygen consumption enzymatic activity assay produces alphaâketoglutarate cellular growth rate substrate binding site haâtagged vectors 100 mm trisâhcl
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Analytics and Tracking {đ}
- Google Analytics
- Google Analytics 4
- Google Tag Manager
Libraries {đ}
- jQuery
- jQuery module (jquery-3.6.0)
Emails and Hosting {âď¸}
Mail Servers:
- nihcesxway.hub.nih.gov
- nihcesxway2.hub.nih.gov
- nihcesxway3.hub.nih.gov
- nihcesxway4.hub.nih.gov
- nihcesxway5.hub.nih.gov
Name Servers:
- dns1-ncbi.ncbi.nlm.nih.gov
- dns2-ncbi.ncbi.nlm.nih.gov
- lhcns1.nlm.nih.gov
- lhcns2.nlm.nih.gov
CDN Services {đŚ}
- Ncbi