
NCBI . NLM . NIH . GOV {
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Title[redir]:
Structural insights into the regulation of PDK1 by phosphoinositides and inositol phosphates - PMC
Description:
3-phosphoinositide-dependent protein kinase-1 (PDK1) phosphorylates and activates many kinases belonging to the AGC subfamily. PDK1 possesses a C-terminal pleckstrin homology (PH) domain that interacts with PtdIns(3,4,5)P3/PtdIns(3,4)P2 and with ...
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Keywords {š}
pdk, domain, insp, ptdinsp, binding, protein, figure, inositol, pubmed, google, scholar, doi, kinase, residues, phosphoinositides, phosphates, structure, affinity, pkbα, dphosphate, membrane, domains, cell, data, hydrogen, fulllength, cells, bonds, lys, alessi, phosphoinositide, pkb, ligand, pmc, homology, shown, complex, pleckstrin, fold, activation, interact, isolated, plasma, observed, interactions, arg, site, currie, biol, article,
Topics {āļø}
defined ā£foā£āā£fc⣠density charcotāmarieātooth syndrome preliminary x-ray diffraction growth factor-induced activation pmc beta search pif-binding docking site p70 ribosomal s6-kinase gst-tagged wild type high-affinity docking site c-terminal α helix pdk1 ph domainādic4āptdins c-terminal pleckstrin homology full-length protein localises d6-hydroxy groups show phosphoinositide-specific phospholipase cdelta4 signal-dependent membrane targeting 4-bisphosphate-binding pleckstrin homology isolated lipid-binding domain dapp1/grp1/btk ph domains 3-phosphoinositide-dependent protein kinase 3-phosphoinositide-dependent protein kinase-1 n-terminal α1ā² helix purified gst-pdk1 species wild-type ph domain free oxygen atoms d6-phosphorylated inositol phosphates quantitative tr-fret analysis protein-lipid overlay assay p-binding phox homology spacious ligand-binding site mutant full-length pdk1 wild-type gst pkbα full-length pdk1 protein n-terminal extension forming displays high-affinity interactions ser/thr residue full-length pdk1 employing phosphoinositide 3-kinase-dependent phosphorylation pdk1[k467a] mutant interacted pleckstrin homology domaināptdins β-sheet hydrogen bonds p3-binding ph domain phospholipid overlay assays d5-phosphate solvent exposed c-terminal domain p3-binding ph domains pdk1 ph domaināptdins px domainādic4āptdins standard ph domain p4-binding ph domains
Questions {ā}
- Irvine R (2004) Inositol lipids: to PHix or not to PHix?
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