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Title[redir]:
Optogenetic Control of Heart Rhythm by Selective Stimulation of Cardiomyocytes Derived from Pnmt+ Cells in Murine Heart - PMC
Description:
In the present study, channelrhodopsin 2 (ChR2) was specifically introduced into murine cells expressing the Phenylethanolamine n-methyltransferase (Pnmt) gene, which encodes for the enzyme responsible for conversion of noradrenaline to adrenaline. ...
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Keywords {🔍}
heart, cells, light, hearts, pnmt, pnmtcrechr, pdcms, cardiac, pacing, doi, optogenetic, expression, mice, rhythm, fig, interval, google, scholar, mouse, pubmed, stimulation, pulses, chr, adult, pmc, study, left, fluorescence, adrenergic, article, cell, tdtomato, cardiomyocytes, free, video, ventricular, sinus, ecg, isolated, tissue, regions, distribution, model, properties, myocytes, specific, supplementary, control, image, single,
Topics {✒️}
semi-automatic intensity-based method pnmt+/cre rosa26+/βgal mice pnmt+/cre rosa26+/βgal mouse anti-α-actinin antibody staining pnmt-cre/chr2 mice provide cell type-specific promoter inducible cre/loxp systems chr2/tdtomato-positive cardiac myocytes ultra-thin microscopy sections pmc beta search intensity-based linear interpolation chr2 light-sensitive channel crossing pnmt-cre mice4 article references data chr2/tdtomato fusion protein crossing pnmt-cre mice phenylethanolamine n-methyltransferase locus latent chr2/tdtomato gene [google scholar] represent chr2/tdtomato fluorescence sarcomeric α-actinin pnmt+ cell-derived cells chr2 light-sensitive channels pnmtcre/chr2 adrenal medulla sympathetic nerve–mediated regulation adult pnmtcre/chr2 hearts chr2/tdtomato-positive cells neural crest-derived cells expressing chr2/tdtomato supplementary materials included free wall ca2+ transients evoked chr2 staining/tdtomato fluorescence anti-α-actinin pnmt-expressing neuroendocrine cells assess global ca2+ neural crest cells light-paced la dissected homozygous rosa26 reporter end-user tool license information pmcid selective cardiac repair showed sinusoidal pattern pnmt-positive pdcms cells effective refractory period primary antibodies overnight pnmtcre/chr2 animals pacemaker cell marker voltage-sensitive dye rh237 representative ecg recordings
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