
NCBI . NLM . NIH . GOV {
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Title[redir]:
Reconstitution of Targeted Deadenylation by the Ccr4-Not Complex and the YTH Domain Protein Mmi1 - PMC
Description:
Ccr4-Not is a conserved protein complex that shortens the 3′ poly(A) tails of eukaryotic mRNAs to regulate transcript stability and translation into proteins. RNA-binding proteins are thought to facilitate recruitment of Ccr4-Not to certain mRNAs, ...
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Keywords {🔍}
ccrnot, doi, mmi, complex, rna, deadenylation, pubmed, google, scholar, figure, pmc, article, activity, recombinant, rnabinding, free, polya, substrate, protein, yth, domain, proteins, rnas, tail, yeast, mrna, subunits, specificity, caf, purified, figures, cell, sequence, expression, biol, mrnas, mol, interaction, vitro, upstream, affinity, mera, region, target, ccr, specific, binding, dsr, active, native,
Topics {✒️}
single-stranded-nucleic-acid-binding capacity intrinsic n-terminal low-complexity region low-complexity n-terminal region intrinsic single-stranded-rna-binding activity ��35-kda amino-terminal low-complexity single-stranded 20-mer-a30 rna gln/asn-rich regions found c-terminal yth domain tap-tagged caf40/rcd1 subunit multi-angle light scattering pmc beta search complementary single-stranded dna mmi1 n-terminal deletions glutamine/asparagine-rich stretches supplemental experimental procedures rna n6-methyladenosine methylation 1× tbe [tris-borate-edta] extensive low-complexity sequences potential rna-binding surface author contributions c-terminal tap tag cis-acting rna elements rna elimination machinery supplemental information mmc2 u-rich-a30 rna contained single-point mutations vitro cre-lox recombination rna-binding protein adapters n6-methyladenosine-dependent regulation additional rna-binding proteins puf protein-mediated deadenylation rna-binding surface contributed highly abundant n6-methyladenosine cell-free system rna-binding adapter protein multiple rna-binding proteins low-complexity regions rna-binding protein mmi1 preventing mmi1-dsr interactions figures s1–s7 mmc1 unstructured 20-mer-a30 substrate target 20-mer-a30 rna entire 20-mer-a30 rna u-rich upstream sequence rna-binding protein essential rna-binding domain found tap tagged yielded including stem cell maintenance low-complexity region license information pmcid
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