
NCBI . NLM . NIH . GOV {
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Title[redir]:
Molecular basis for cytoplasmic RNA surveillance by uridylationâtriggered decay in Drosophila - PMC
Description:
The posttranscriptional addition of nucleotides to the 3Ⲡend of RNA regulates the maturation, function, and stability of RNA species in all domains of life. Here, we show that in flies, 3Ⲡterminal RNA uridylation triggers the processive, 3â˛âtoâ5Ⲡ...
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Keywords {đ}
rna, dmdisl, fig, decay, uridylation, data, tailor, doi, google, scholar, pubmed, substrates, pmc, cells, free, article, rnase, degradation, flies, substrate, analysis, end, complex, trna, mrna, terminal, transcripts, figure, disl, cell, drosophila, control, trump, posttranscriptional, species, polymerase, source, total, shown, determined, uridine, file, sequencing, protein, interaction, function, exoribonuclease, small, click, additional,
Topics {âď¸}
misâlocalized lsmâclass snrna pagwâtailor encoding fullâlength [google scholar] su 625 nm Îąâ32pâutp pmc beta search mg2+âdependent nucleophilic attack epitopeâtagged catalyticâmutant dmdis3l2 Îąâ32pâutpâincorporation signals ďż˝terminal coiledâcoil motif singleâstranded rnaâspecific exoribonuclease predicted coiledâcoil motif effective free energy induce replicationâdependent decay highâthroughput sequencing reveals mouse antiâflag m2 multiple uracilâspecific interactions bacterial/archaeal/organellar polyadenylation highâthroughput sequencing datasets singleâstranded landing site rnaâspecific ribonucleotidyl transferases expressing flagâtagged tailor crispr/cas9 genome editing target rnaâdirected trimming included flagâtagged gfp employing Îąâ32pâatp amino acidâexchange mutation crispr/cas9 genome engineering highâthroughput sequencing revealed singleâexponential decay kinetics tho/sub2 complex mutants templateâindependent terminal ribonucleotidyltransferases recent highâthroughput methods flagâdmdis3l2 recovered gfpâtailor singleâexponential reaction kinetics highâthroughput biochemical assay singleâstranded entry point singleâstranded rna ending triggering dmdis3l2âdirected decay initiate dmdis3l2âdirected decay highâthroughput biochemical characterization highâthroughput biochemical comparison gfpâtailor fullâlength 32pâlabeled rna species uridylationâtriggered rna decay rabbit antiâgfp antibody singleâstranded rna extension heso1âdirected uridylation tailorâdirected rna uridylation singleâstranded rna substrates wildâtype s2 cells
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