
NCBI . NLM . NIH . GOV {
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Title[redir]:
AMPK regulates autophagy by phosphorylating BECN1 at threonine 388 - PMC
Description:
Macroautophagy/autophagy is a conserved catabolic process that recycles cytoplasmic material during low energy conditions. BECN1/Beclin1 (Beclin 1, autophagy related) is an essential protein for function of the class 3 phosphatidylinositol 3-kinase ...
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Keywords {🔍}
becn, autophagy, cells, ampk, phosphorylation, glucose, fig, hekt, pmid, doi, pubmed, google, scholar, httpdxdoiorg, cell, prkaa, gstbecn, thr, pikc, pmc, starvation, kinase, antibody, protein, complex, article, bcl, proteins, domain, free, mutant, detected, interaction, endogenous, deprivation, results, transfected, expressing, atg, formation, encoding, mef, gst, biol, activity, phosphorylated, puncta, antibodies, glutathione, mutants,
Topics {✒️}
mouse embryonic fibroblast methods abbreviations disclosure amp-activated protein kinase bcl-xl-beclin1 peptide complex beclin1-phosphatidylinositol-3-kinase complex plasmid encoding gfp-prkaa1-ca full-length becn1 protein anti-phospho-becn1 t388 antibody 3′ untranslated region constructs encoding gfp-prkaa1-wt protein serine/threonine kinase flag-becn1t388d-expressing stable cells pik3c3-becn1-atg14 complex formation kap1-mediated vps34 sumoylation unique autophagy-related protein 50 μg/ml penicillin/streptromycin final concentration 1 μg/μl pmc beta search actb/β-actin antibodies pan-hdac inhibitor panobinostat plasmid expressing gfp-bcl2 plasmids encoding gfp-prkaa1wt plasmid expressing myc-bcl2 central coiled-coil domain plasmid expressing gfp-atg14 β-α repeated phospho-prkaa/ampkα t172 wild-type flag-becn1 plasmid encoding gfp-atg14 dap-kinase-mediated phosphorylation plasmid encoding gfp-lc3 kinase dead prkaa1 immunoprecipitated flag-becn1t388a-expressing cells p-becn1 t388 antibody p-ampk substrate antibody n-terminal region plasmids expressing gst-becn1 becn1t388a-pik3c3 complex formed coexpressed gfp-prkaa1-ca becn1 t388-induced autophagy plasmid encoding gst-becn1wt beclin1-binding protein uvrag vacuolar protein sorting plasmids expressing gst-becn1wt becn1-bcl2 complexes formed constitutively active plasmids encoding flag-becn1wt pik3c3-becn1-atg14 complex plasmids encoding gst-becn1wt serine/threonine kinase
Questions {❓}
- Then, a question arises: how does Thr388 in the BARA domain influence both the BH3 domain (BCL2 binding domain) and CCD (ATG14 binding domain)?
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Analytics and Tracking {📊}
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Emails and Hosting {✉️}
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