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Keywords {🔍}

caspase, ilβ, caspasegyrase, cell, cells, figure, death, doi, pubmed, google, scholar, secretion, dimerization, pmc, inflammasome, mefs, treated, article, gfp, free, activation, proilβ, cleavage, supplementary, nlrp, coumermycin, supernatants, dimerized, expression, release, western, elisa, active, absence, doxycycline, μgml, blot, experiments, independent, released, protein, induce, data, supernatant, elispot, usa, tnf, lysates, nfκb, processing,

Topics {✒️}

pan-caspase inhibitor z-vad-fmk full-length mouse il-1β gfp-expressing pro-il-1β construct caspase-1-mediated interleukin-1β activation francisella-infected caspase-1-deficient macrophages fused c-terminal gyrase-gfp real-time single-cell imaging pro-il-1β gfp constructs mediates noncanonical il-1beta directly process pro-il-1β 25 μm z-vad-fmk propidium iodide n-terminal flag tag pmc beta search nf-κb gfp reporter ptrh1-nf-κb-dscgfp nf-κb reporter construct caspase-8−/− mefs-expressing caspase-1-gyrase expresses pro-il-1β murine sv40 large produce mature il-1beta pro-interleukin-1beta cellular flice-inhibitory protein tumor necrosis factor similar nf-κb activity time mature il-1β il-1 beta-converting enzyme gfp-tagged dimerizable doxycycline overexpressed pro-il-1β recombinant mature il-1β mature il-1β leaves treat auto-inflammatory diseases caspase-1-gyrase fusion protein blocking il-1β signaling inactive pro-il-1β pro-il-1β constructs wild-type caspase-1-gyrase study caspase-1-mediated activation directly cleaving il-1β z-vad-fmk il-1β-activating platforms cleave pro-il-1β indicating nf-κb activation mouse embryonic fibroblasts induce tnfalpha-dependent apoptosis activate il-1β directly directly activate il-1β pro-il-1β cleavage prevented il-1β cleavage cells secreting il-1β

Questions {❓}

• Ambiguities in NLRP3 inflammasome regulation: is there a role for mitochondria?

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