
NCBI . NLM . NIH . GOV {
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Title[redir]:
Improved Identification and Analysis of Small Open Reading Frame Encoded Polypeptides - PMC
Description:
Computational, genomic, and proteomic approaches have been used to discover non-annotated protein-coding small open reading frames (smORFs). Some novel smORFs have crucial biological roles in cells and organisms, which motivates the search for ...
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Keywords {🔍}
doi, seps, sep, google, scholar, pubmed, figure, pmc, peptide, proteins, database, article, cells, free, methods, acid, data, cell, protein, peptides, smorfs, proteomics, human, detected, samples, buffer, mass, extraction, nonannotated, total, analysis, smorf, column, hcd, sequence, discovery, identified, enrichment, lcmsms, lysis, min, rnaseq, msms, results, kda, uniprot, spectra, acetic, sample, number,
Topics {✒️}
high-energy collisional dissociation collision induced dissociation automatic gain control molecular weight cutoff heme oxygenase 1 solid-phase extraction resulting smorf-encoded polypeptides mass spectrometry approaches collision energy detect smorf-encoded polypeptides sds-page gel lumbar region mass spectrometry resulting lc-ms/ms dataset high-resolution ms/ms data label-free lc-ms lc-ms/ms datasets lc-ms/ms optimization annotated protein-coding orfs lc-ms/ms analysis lc-ms/ms proteomics high agc settings cutoff 5’utr 3’utr protein-coding sorf acquired ms/ms spectra ms/ms spectra acquired lc-ms/ms fusion tribrid ms hcd energy high-resolution hcd high resolution hcd label-free sep quantitation reverse phase enrichment qe mass spectrometer label-free quantitation method exactive mass spectrometer 30-kda mwco resulted seps ms/ms spectra savant speed-vac concentrator rabbit anti-ho-1 30-kda mwco filter disease protein v642i-app sds-page reveals ca014195 mass core soc mass spectrom high background noise lc-ms peaks lc-ms peak
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