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Keywords {🔍}

nsun, mitochondrial, rna, supplementary, cells, fig, doi, google, scholar, pubmed, pmc, article, human, free, reads, mttrnamet, miclip, data, rnaseq, methylation, mutmut, protein, patient, performed, sequencing, analysis, wtwt, fibroblasts, position, translation, dna, detected, mutations, cytosine, previously, modification, cell, mtdna, mitochondria, levels, ref, modifications, expression, mutmutnsun, formation, cdna, min, gene, trnas, hdfs,

Topics {✒️}

hrp-conjugated goat anti-rabbit [google scholar] langmead t7-transcribed radioactive rna-probes methionine/cysteine-free dmem medium mt-trnamet-specific rna-seq reads 3/v5-topo vector system methionine/cysteine-free dmem european genome-phenome archive patient-derived nsun3-null cells s-adenosylmethionine-dependent methyltransferases mitochondrial dna-encoded wild-type pcmv6-entry-myc vector pmc beta search full-length nsun3 cdna bs rna-seq approaches redbs rna-seq results full-length nsun3 protein mitochondrial asparaginyl-trna synthetase nsun3-v5 expression constructs v5-tagged nsun3 construct fcab rna-seq results bicarbonate-free dmem full-length nsun3 mrna mouse anti-flag igg high-confidence methylation sites bs rna-seq reads illumina miseq/hiseq platform mut/mut sample excised flag-tagged nsun3 served nsun3-v5 expression construct engineered zinc-finger proteins redbs rna-seq data nsun3 miclip-mutant constructs bs rna-seq method e-rare project genomit hits-clip read counts flag-tagged nsun3 construct fast gapped-read alignment mediates myc-induced proliferation hits-clip-detected sites bs rna-seq confirm fcab rna-seq reads site-directed mutagenesis nuclear-encoded factors involved post-transcriptional modification patterns high-throughput sequencing reads express nsun3-flag-strep2 additional information vivo isotope-tracing experiment nsun3-null patient fibroblasts

Questions {❓}

• How does the lack of NSun3-mediated mt-tRNAMet C34 methylation affect mitochondrial translation?
• Why has m5C34 in mt-tRNAMet not been detected in previous studies, despite relatively high detection levels in BS RNA-Seq?

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