
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Identification of selective inhibitors of cancer stem cells by high-throughput screening - PMC
Description:
Screens for agents that specifically kill epithelial cancer stem cells (CSCs) have not been possible due to the rarity of these cells within tumor cell populations and their relative instability in culture. We describe here a novel approach to ...
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Keywords {🔍}
cells, cancer, treatment, breast, salinomycin, doi, cell, paclitaxel, figure, cscs, epithelial, fold, compounds, hmler, google, scholar, pubmed, tumor, gene, expression, emt, populations, relative, genes, tumors, increased, mammary, human, hmleshecad, control, stem, csc, pmc, drug, increase, agents, toxicity, proportion, resistance, treated, compound, mice, days, cdhighcdlow, shown, hmlershecad, data, article, ecadherin, set,
Topics {✒️}
human colon-cancer-initiating cells pgc-1alpha-responsive genes involved cd44high/cd24low csc-enriched subpopulations [google scholar] ricci-vitiani pe-conjugated anti-cd24 antibody histology-special procedures lab cd44hi/cd24lo marker profile clones ae1/ae3&pck26 pmc beta search high-resolution mass spectrometry cd44high/cd24low marker profile p53-null mouse model fluorescence-activated cell sorting normalized replicate z-scores patient-derived breast cancers apc-conjugated anti-cd44 low/high signal intensities cd44high/cd24low antigenic phenotype cell-surface marker profiles hmler-shecad cells grown high-throughput screening piyush tumorigenic breast-cancer cells neoplastic stem-cell states epithelial csc-specific toxicity trypan blue dye-exclusion 4t1 populations pre-treated cd44high/cd24low hmler_mx cells broad-spectrum kinase inhibitor hmle-shecad cell strains cd44high/cd24low cells relative increased tumorsphere-forming ability 4t1 cells pre-treated generating dose-response curves sum159 tumor-growth curves increased tumor-seeding potential high-throughput screening method tumorsphere-forming cells relative shrna targeting e-cadherin immortalized mammary epithelial hmler_mx populations compound-treated ultra-low attachment plates hmler cells compound-treated compound/cell line combination cd44high/cd24low cells quantified metastatic nodule-forming ability e-cadherin promotes metastasis hmle-shecad cells treated 4t1 metastasis-bearing animals xenograft tumor-seeding studies human breast cancers
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