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Title[redir]:
A Novel [15N] Glutamine Flux using LC-MS/MS-SRM for Determination of Nucleosides and Nucleobases - PMC
Description:
The growth of cancer cells relies more on increased proliferation and autonomy compared to non-malignant cells. The rate of de novo nucleotide biosynthesis correlates with cell proliferation rates. In part, glutamine is needed to sustain high rates ...
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Keywords {๐}
positive, glutamine, doi, nitrogen, figure, nucleobases, dna, nucleosides, cancer, scholar, mass, pubmed, google, cells, ion, adenosine, flux, cell, synthesis, adenine, spectrometry, glutamate, pmc, srm, min, cytosine, guanosine, cytidine, thymidine, uridine, shows, nlm, bladder, acid, guanine, thymine, uracil, spectrum, incorporation, proliferation, pyrimidine, set, amino, open, tab, ions, ncbi, search, nucleotide, biosynthesis,
Topics {โ๏ธ}
cell type-dependent manner liquid chromatography-mass spectrometry gas chromatography-mass spectrometry dpbs-1x-corning cat rrhd sb-cn column pmc beta search metabolomic core facility pmc copyright notice acetonitrile/trifluroacetic acid/water lc-ms/ms-srm mass spectrometry analysis publisher site pdf cell proliferation rates high throughput analysis primary nitrogen source license information pmcid american cancer society reverse phase chromatography phosphate buffered saline de novo synthesis cell accelerator voltage cid mass spectrum anal bioanal tech sustain high rates cancer cells relies prostate cancer cells glutamine amido โnitrogenโ cambridge isotope labs stable-isotope tracers low pressure rf duncan cancer center key enzymes involved selected reaction monitoring accelerated aerobic glycolysis nonessential amino acid qiagen dneasy blood 1m ammonium acetate 1m ammonium bicarbonate speed vacยฎ system van der berg hippocampal slices induced product-ion spectrum labeled nitrogen incorporated clin cancer res nitrogen drying gas nitrogen nebulizing gas ๏ฟฝ๏ฟฝ2nch=nh]+ ion analysis nitrogen flux importantly proliferating cells sheath gas flow
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