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Title[redir]:
Impaired Bub1 Function In vivo Compromises Tension-Dependent Checkpoint Function Leading to Aneuploidy and Tumorigenesis - PMC
Description:
Bub1 is a serine/threonine kinase originally described as a core component of the spindle assembly checkpoint (SAC) mechanism in yeast. Bub1 binding at kinetochores has been reported to be required for SAC function and localization of other SAC ...
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Keywords {🔍}
bub, doi, mefs, bubδδ, cells, checkpoint, sac, pubmed, google, scholar, spindle, mice, cell, bubr, chromosome, mitotic, bubδ, kinetochore, protein, mad, localization, allele, levels, function, mutant, aneuploidy, response, nocodazole, pmc, tumor, fig, article, cancer, chromosomes, senescence, treatment, free, kinetochores, animals, background, reduced, increased, congression, high, results, mutation, taxol, anaphase, loss, genetic,
Topics {✒️}
fvb-β-actin-cre deleter strain x-linked cmv-cre strain splice-acceptor-β-geo cassette mad3/bub1-related protein kinase supp 2 nihms761127-supplement-supp_2 supp 3 nihms761127-supplement-supp_3 supp 4 nihms761127-supplement-supp_4 anti–phosphorylated histone h3 phosphorylated histone h3-positive nci 2-r01-ca065773-11a1 β-galactosidase assay buffer loxp-flanked zeocin cassette pmc beta search serine/threonine kinase originally β-galactosidase assays revealed cre-mediated cassette removal 129/bl6 bub1δ2-3/δ2-3 mefs bub1δ2-3/δ2-3 mefs exhibit bub1δ2-3/δ2-3 mefs suggested correctly targeted clones quantitative reverse transcription–pcr bub1δ2-3/δ2-3 cultures contained taxol-treated bub1δ2-3/δ2-3 mefs bub1δ2-3/δ2-3 mice die bub1δ2-3/δ2-3 mefs grew sister chromatid cohesion neomycin resistance cassette gene-trapped allele xg453 es cells β-geo cassette nocodazole-arrested mefs identified bub1δ2-3/δ2-3 embryos led unperturbed bub1δ2-3/δ2-3 mefs immunoprecipitation–western blot analysis bub1 gene-trap allele persistent cohesion site bub1δ2-3/δ2-3 mefs expressed phosphorylated h2ax immunostaining early postnatal period nh2 terminal domain bub1-mediated inhibitory phosphorylation carcinogen-induced tumor formation serine/threonine kinase anti–β-tubulin 129p2/olahsd strain mouse embryonic fibroblasts integrated signaling system spindle checkpoint machinery c57bl/6j blastocysts severe defects ranging
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