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Title[redir]:
Simultaneous generation of many RNA-seq libraries in a single reaction - PMC
Description:
Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, ...
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Keywords {🔍}
doi, pubmed, google, scholar, samples, pmc, rna, article, free, reads, supplementary, rnatagseq, library, gene, adaptors, genes, barcodes, methods, rnaseq, expression, total, coli, barcode, mouse, padj, libraries, barcoded, fig, construction, data, nat, guttman, generated, number, table, cell, time, protocol, sequencing, read, adaptor, analysis, science, single, sequence, identified, genome, nlm, nmeth, approach,
Topics {✒️}
refseq-annotated protein-encoding genes protein-rna interaction mapping pmc beta search mid-exponential lb cultures initial rnatag-seq protocol rnatag-seq cdna libraries generated rna-seq libraries enabling strand-specific competing financial interests single rna-seq library nat methods abstract rna-seq data generated rna-seq library construction david web service34 publisher site pdf single-sample library construction normalized read counts refseq reference genomes source data files rna-seq libraries illumina cdna libraries quality highly comparable muriel jacobs genetics license information pmcid rna level patterns differential expression analysis small rna-seq united states government final edited form important conceptual advance benjamini-hochberg procedure clinically relevant pathogens dna-damaging agents archived formalin-fixed institutional review board mueller-hinton broth maximal amount recommended single-stranded cdna refseq accession nc_009800 early independence award bacterial dutp libraries report rnatag-seq rnatag-seq data illumina sequencing adaptors sequencing error correction barcoded rna samples13–17 illumina index barcodes generate cdna libraries rnatag-seq protocol numerous libraries generated
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