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Title[redir]:
Human Haploid Cell Genetics Reveals Roles for Lipid Metabolism Genes in Nonapoptotic Cell Death - PMC
Description:
Little is known about the regulation of nonapoptotic cell death. Using massive insertional mutagenesis of haploid KBM7 cells we identified nine genes involved in small-molecule-induced nonapoptotic cell death, including mediators of fatty acid ...
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Keywords {🔍}
cell, cells, death, doi, cil, pubmed, google, scholar, pmc, figure, article, free, genes, ferroptosis, fatty, acc, rsl, compounds, kbm, lethal, biol, nonapoptotic, stockwell, identified, chem, acid, insertions, gene, significantly, acids, lipid, system, analysis, acaca, nlm, results, genetic, small, gpx, erastin, abt, supplemental, tofa, nat, haploid, acsl, lpcat, data, clones, dna,
Topics {✒️}
phorbol-12-myristate-13-acetate-induced protein 1 acetyl-coa carboxylase alpha false-discovery rate corrected retroviral-mediated insertional mutagenesis pmc beta search 1-acylglycerol-3-phosphate o-acyltransferase 3 additional microtubule-disrupting agents fatty acid-induced lipotoxicity sds-polyacrylamide gel electrophoresis oxidation-sensitive fatty acids general drug-resistant phenotype dmem high-glucose media reducing agent beta-mercaptoethanol cil56-induced cell death apoptosis-inducing agent pk-3-resistant colonies yielded 18 tetradecyloxy-2-furonic acid rate-limiting step iron-dependent form acc1-dependent production striking tofa-sensitive accumulation crispr/cas9 technology cell death induced short guide rnas haploid genetic screens li-cor biosciences mct1-mediated transport nonapoptotic cell death supplementary material cb5b00245_si_001 p53-mediated activity small molecule lethality publisher site pdf protease inhibitor tablet ml162-resistant cells identified haploid kbm7 cells plasmid encoding cas9 rsl3-resistant cells identified death-inducing compounds regulated cell death massive insertional mutagenesis polyunsaturated fatty acids saturated fatty acids fatty acid synthesis fatty acid synthase retroviral insertion sites detoxify lipid peroxides blocking buffer overnight mammalian reductases involved saturated fatty acids25 taq dna polymerase
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