
NCBI . NLM . NIH . GOV {
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Title[redir]:
N6-methyl-adenosine (m6A) marks primary microRNAs for processing - PMC
Description:
The first step in the biogenesis of microRNAs is the processing of primary microRNAs (pri-miRNAs) by the microprocessor complex, composed of the RNA binding protein DGCR8 and the ribonuclease type III DROSHA1–4. This initial event requires the ...
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Keywords {🔍}
mettl, rna, doi, cells, fig, primirna, processing, dgcr, mirnas, data, expression, buffer, primirnas, extended, control, mirna, pubmed, google, scholar, pmc, analysis, depletion, vitro, sequences, qrtpcr, min, represent, motif, levels, article, nature, mature, cell, hitsclip, experiments, free, figure, bar, scale, immunoprecipitation, extracted, open, tab, linear, quantification, prilete, normalized, shown, methylated, nuclear,
Topics {✒️}
tbe-urea poly-acrylamide gel fast gapped-read alignment c-terminal flag tag cross-linking induced mutations 50bp single-end runs anti-digoxigenin-ap antibody key post-transcriptional modification flanking single-stranded rna pri-mirna sequences initiates intragenic pri-mirna sites taqman pri-mirna assay 1038/nature14281 n6-methyl-adenosine mettl3 hits-clip tags cell-type specific manner pmc beta search lower panel pri-mir-1-1 produce pre-let7e relative promoting pri-mirna processing pri-mirna processing reactions mda-mb-231 cell lines α-mettl3 rabbit antibody rabbit α-m6a antibody pri-mirna sequences relative mettl3-dependent m6a modification conducted high-throughput sequencing α-dgcr8 rabbit antibody mda-mb-231 cells expressing radio-labeled rna linker linear scale heat-map dig-labeled lna probes pre-mirna genomic loci license information pmcid pri-mirna genomic regions vitro transcribed pri-mir-1-1 vivo protein-rna interactions magnetic bead bound-dgcr8 mirna control pri-mir-1-1 microrna expression profiling mettl3-interacting protein dgcr8 rnase-free water publisher site pdf impact pri-mirna levels vitro transcribed pri-mirnas primary microrna processing hits-clip tags hits-clip data performed qrt-pcr reactions taqman microrna assays strand synthesis system hits-clip protocol
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