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Keywords {🔍}

doi, mrna, rna, google, scholar, pubmed, methylation, mrnas, pmc, article, fto, free, studies, cell, adenosine, residues, cells, methylated, mettl, proteins, protein, binding, cellular, rnas, specific, analysis, methyltransferase, meripseq, peaks, sites, found, nuclear, biol, base, mammalian, function, levels, complex, alkbh, target, nmethyladenosine, showed, demethylation, expression, modification, pathways, splicing, important, transcript, transcripts,

Topics {✒️}

targetscan-predicted microrna-binding site trans-acting tissue-specific factors single-molecule real-time detection exhibit tissue-specific differences4 controlling nonsense-mediated decay pmc beta search mammalian wilm's-tumor-1 microrna-induced mrna silencing specific trans-acting factors high-affinity m6a-binding proteins brain-enriched microrna-binding sites watson-crick base pairing thin-layer chromatography [tlc] tissue-specific expression patterns blocking rna—protein interactions vivo protein-rna interactions alkbh5-knockout mice grow par-clip studies probing yth rna-binding domain87 undergo post-transcriptional methylation published merip-seq datasets3 rous sarcoma virus merip-seq involves immunoprecipitation m6a-sensitive ligase reaction merip-seq dataset show m6a-binding ribonucleoprotein complex oligonucleotides possessing n6-methyladenosine identifying cis-acting elements pre-immunoprecipitation rna fragments putative m6a-binding protein n6-methyladenosine-dependent regulation merip-seq dataset revealed rna n6-methyladenosine methyltransferase high-resolution mapping reveals supplementary materials included putative m6a-binding proteins protein-rna binding affinity merip-seq data shows facilitating rna—protein interactions microrna-binding sites suggests compartment-specific m6a roles rna-binding protein hur methylation state-specific recognition fto-knockout brain tissue pre-mrna processing events merip-seq analysis shows merip-seq data showed fto-homolog tet proteins84 induce rna—protein interactions ribosome-mediated methylation pathway

Questions {❓}

• Could other methyltransferases drive m6A formation?
• Do these proteins bind m6A in living cells?
• Does protein synthesis occur in the nucleus?
• How is methylation targeted?
• The presence of such a short methylation motif raises the question why so few are methylated in mRNAs?
• What is the difference between these two FTO and ALKBH5?
• What kind of binding selectivity can be achieved with such a small modification like a methyl group?
• Where in the cell does methylation occur?

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