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Title[redir]:
Yeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1 - PMC
Description:
The function of RNA is subtly modulated by post-transcriptional modifications. Here, we report an important crosstalk in the covalent modification of two classes of RNAs. We demonstrate that yeast Kre33 and human NAT10 are RNA cytosine ...
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Keywords {🔍}
doi, rrna, nat, yeast, figure, rna, cells, google, scholar, kre, pubmed, acetylation, human, trna, modification, pmc, article, free, required, cell, protein, subunit, min, helix, total, buffer, supplementary, domain, small, ribosome, analysis, biogenesis, depletion, modifications, processing, trnas, analyzed, prerrna, function, efficient, ribosomal, helicase, mol, hplc, acetylated, nucleolar, involved, purified, shown, tan,
Topics {✒️}
horseradish-peroxidase-conjugated anti-rabbit igg horseradish-peroxidase-conjugated anti-mouse igg gsk-3beta-regulated n-acetyltransferase 10 high-fidelity pfu-dna polymerase watson–crick base-pair complementarity rna-polymerase-i-dedicated modification low-molecular-weight rna species n-1-methyl-adenosine base modification optima l-100xp ultracentrifuge premature-aging progeria syndrome 5-ml antibiotic-free medium high-molecular-weight species 2d thin-layer chromatography pmc beta search human glyceraldehyde-3-phosphate dehydrogenase acetyl-coa synthetases acs1 open-source software t-coffee watson–crick base pairing uv cross-linker cl-508 dans l'agriculture [ pre-rrna intermediates established acetyl-coa binding pocket 90s pre-ribosomes include isogenic wild-type control a-site finger region ice-cold glass beads de la cruz numerous post-translational modifications early pre-rrna processing acetyl-coa binding domain 20-mm tris-hcl ph7 pgal-3ha-kre33 strain efficient site-directed mutagenesis mung bean nuclease 500-μl ice-cold acetone post-transcriptional modifications highlighted pre-ribosomal rna processing p53 post-translational modification helicase-deficient mutant k289a rna–dna hybrid forms atpase-dependent helicase function nat10-induced nucleolar stress rapid trna decay ice-cold 1x pbs small-subunit ribosomal rna complete protease inhibitor pre-rrna processing analysis efficient pre-rrna processing rnase-free dnase telomere length regulation
Questions {❓}
- How does Kre33 or NAT10 recognize its substrates?
- What are the functions of 18S rRNA and tRNA acetylation?
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Analytics and Tracking {📊}
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