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🌠 Phenomenal Traffic: 5M - 10M visitors per month


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Keywords {🔍}

dna, protein, diffusion, sliding, target, google, scholar, site, pubmed, doi, binding, rate, time, length, sites, proteins, association, distance, targeting, nonspecific, specific, lac, dissociation, size, interactions, processivity, probability, random, repressor, number, radius, find, reaction, constants, coil, biol, experiments, facilitated, occur, search, pmc, long, large, constant, lsl, dnabinding, rates, motion, molecule, molecules,

Topics {✒️}

tus-ter protein-dna interaction dye-impregnated colloidal particles lac repressor-operator interaction lac repressor–operator system lac repressor-operator system lac repressor-operator association pmc beta search diffusion-controlled protein-dna association enzyme-mediated dna looping rna polymerase-dna interactions cold spring harbor site-specific dna-binding protein escherichia coli system protein–ligand associations involve transcription factor-dna interaction power law behaviour larger inter-site spacings diffusion-controlled macromolecular interactions specific dna–protein interaction increasing inter-site spacing coiled-chain macromolecules applied nucleic acid res dimeric mutant laciadi cornell university press integration host factor diffusion-limited reaction rate winding positive supercoils rna polymerase binding yeast gal4 protein dna site-specific targeting escherichia coli cell dimensional diffusion-limited targeting repressor-operator association specific dna–protein interactions dna-binding event relative tata binding protein rna polymerase sliding replication terminator tus dna–protein association rates protein–dna association rates revealed large variations processive dna–protein interactions individual protein–dna interactions princeton university press cambridge university press site-specific binding reactions repressor-operator recognition base pair step lac repressor tetramer specific interaction strength

Questions {❓}

• Given an initial distance r between protein and target, which must be considerably larger than either a or d (considering the nM concentrations relevant for site-specific binding reactions, it is useful to bear in mind that 1 nM corresponds to 1 molecule/µm3 or one molecule per Escherichia coli cell), what is the probability that binding will occur via simple diffusion?
• HOW FAST DO PROTEINS ACTUALLY BIND TO SPECIFIC DNA SITES?
• How do site-specific DNA-binding proteins find their targets?

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