
NCBI . NLM . NIH . GOV {
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Title[redir]:
Impairment of chaperone-mediated autophagy leads to selective lysosomal degradation defects in the lysosomal storage disease cystinosis - PMC
Description:
Metabolite accumulation in lysosomal storage disorders (LSDs) results in impaired cell function and multi-systemic disease. Although substrate reduction and lysosomal overload-decreasing therapies can ameliorate disease progression, the significance ...
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Keywords {π}
ctns, cells, lysosomal, doi, lampa, cma, cystinosis, pubmed, google, scholar, cell, degradation, lysosomes, autophagy, cystinotic, increased, cuervo, fibroblasts, mice, data, levels, pmc, lamp, figure, impaired, article, supplementary, free, expression, analysis, membrane, substrate, autophagosomes, cystine, number, mouse, results, activity, autophagic, overload, proteins, show, autophagosome, treatment, starvation, kaushik, analyzed, localization, ctnsdeficient, cysteamine,
Topics {βοΈ}
clathrin-independent vamp7/vps41-mediated transport anti-oxidant drug n-acetylcysteine n-linked carbohydrate-independent mechanism ctnsβ/β-gfp-lc3 transgenic mice pmc beta search nih grants ro1-dk090058 fresh serum-free dmem tandem fluorescent-tagged lc3 national bio-resource project ubiquitin-binding scaffold protein regulates chaperone-mediated autophagy comparing lc3-ii amounts gfp-positive structures relative c57bl/6 ctnsβ/β gfp-lc3+/+ measuring Ξ²-hexosaminidase activity unique carboxy-terminal domain serum-free dmem edta-protease inhibitors cocktail gfp/rfp-positive structures gfp-ctns expression led lc3b-ii levels depending bone marrow-derived macrophages chaperone-mediated autophagy leads ctns-deficient cells resulted lysosomal overload-decreasing therapies tab chaperone-mediated autophagy endogenous lc3b-positive structures rab11-positive trafficking vesicles long-lived protein degradation transgenic mice expressing induced lc3b-ii degradation akt/mtor signaling pathway lysosomal overload-decreasing therapy anti-lamp2a abcam ab18528 performed quantitative rtβpcr long-lived proteins degradation wong l-jc evaluated lysoso-mal localization impaired chaperone-mediated autophagy lamp1-negative structures observed cysteamine-cysteine mixed disulfide n-acetyl-cysteine autophagosome marker lc3b-ii vamp7-dependent transport system amino acid/serum starvation tab ctns-deficient cells post-translational modifications anti-lamp2a antibody raised normal growth medium cysteamine-treated ctnsβ/β fibroblasts
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