
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Techniques to Monitor Glycolysis - PMC
Description:
An increased flux through glycolysis supports the proliferation of cancer cells by providing additional energy in the form of ATP as well as glucose-derived metabolic intermediates for nucleotide, lipid, and protein biosynthesis. Thus, glycolysis ...
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Keywords {🔍}
doi, cells, glucose, cell, google, scholar, glycolysis, pubmed, glycolytic, media, flux, pmc, uptake, metabolic, ecar, cancer, metabolite, analysis, activity, article, lactate, metabolites, measurements, free, nbdg, pyruvate, pathway, add, sigma, atp, protocol, min, hexokinase, metabolism, plate, culture, time, extraction, measurement, seahorse, detection, journal, methods, proliferation, data, measure, buffer, nature, enzymes, kinase,
Topics {✒️}
13cflux2—high-performance software suite pre-ischaemic aminooxyacetate loading pmc beta search 2-deoxy-d-[1-14c]-glucose long-chain fatty acids [google scholar] g6pd converts glucose-6-phoshate price [u-13c6]-glucose tumor-induced gene expression vander heiden mg 2-deoxy-d-glucose metabolism rate-limiting glycolytic enzymes main rate-limiting enzymes glucose-derived metabolic intermediates 13c-metabolic flux analysis 13c-glucose-derived carbons lc/esi-ms analysis fluoro-d-glucose nadp+/nadph-dependent enzymes glucose-independent glutamine metabolism nadp+/nadph-dependent enzyme represent valuable targets marin-valencia short half-life steady-state level hplc grade chloroform additional metabolic processes human adp-dependent glucokinase glucose-free radioactive 2-deoxy-d-[1 nuclear magnetic resonance nih grants gm073981 require expensive equipment glioblastomas remain dependent publisher site pdf support cell proliferation [14c]-2-dg uptake locasale jw 2-dexoxy-d-glucose pmc disclaimer rapidly dividing cells suspension-cultured mammalian cells 13c-glucose experiments glucose analog uptake cold h2o solution human metabolic network isotopic tracer experiments supplies biosynthetic intermediates promote tumor growth lc/esi-ms
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