
NCBI . NLM . NIH . GOV {
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Title[redir]:
Serine catabolism regulates mitochondrial redox control during hypoxia - PMC
Description:
The de novo synthesis of the non-essential amino acid serine is often upregulated in cancer. In this study we demonstrate that the serine catabolic enzyme, mitochondrial serine hydroxymethyltransferase (SHMT2) is induced when Myc-transformed cells ...
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Keywords {🔍}
shmt, doi, cells, cell, hypoxia, pubmed, google, scholar, expression, figure, serine, cancer, pmc, tumor, mitochondrial, article, free, data, hypoxic, hifα, kelly, induction, control, neuroblastoma, phgdh, normoxia, myc, exposed, ros, samples, nadph, hif, redox, metabolism, human, measured, pdf, death, levels, hours, nihmssupplementpdf, synthesis, mitochondria, cellular, supplementary, analysis, min, oxygen, pyruvate, contributes,
Topics {✒️}
lentivirus expressing plenti-giii-cmv-shmt2 plenti-giii-cmv-shmt2 plasmid mitotracker red cm-h2ros lower glutathione/glutathione disulfide synergy hydro-rp column containg 20µm d-2hg metabolite profiling identifies 2µg pcdna-c-myc pmc beta search cellular nadph/nadp+ ratio cells expressing c-myc hypoxia-inducible factor-1 α mouse anti-hif1-α athymic nu/nu mice biotinylated goat anti-rabbit 4mm u-13c-serine lentiviruses carrying plko-shshmt2 cellular gsh/gssg ratios mitochondrial complex iii n-myc amplified neuroblastoma kaplan-meier survival analysis sirna targeting c-myc n-methyl-n-tertbutyldimethylsilyltrifluoroacetamide de novo synthesis sh-sy5y cells conferred shrna-expressing virus measured nadph/nadp+ ratio concurrent methylene-thf generation sh-sy5y cell line oxygen-regulated α subunit hif-1-dependent shmt2 induction oxygen-regulated control elements 5 µm cm-h2dcfda sirna targeting hif-1α pyruvate kinase m2 ventana medical systems nadph/nadp+ ratio tumor oxygenation predicts characterized tissue microarray hypoxia-inducible factor 1 promote serine-dependent myc binding sites nucleic acid synthesis shmt2-dependent production pyruvate dehydrogenase kinase u-13c-glycine generated 3µg total rna serine catabolic flux hif-1α staining correlate flow rate 200µl/min
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