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Keywords {🔍}

cells, doi, single, protein, figure, suntag, cell, gene, activation, pubmed, google, scholar, imaging, expression, peptide, gfp, copies, transcriptional, fluorescence, molecule, multiple, pmc, system, proteins, movie, transcription, binding, microtubule, molecules, article, free, array, microscopy, antibody, fused, imaged, results, signal, sgrnas, scaffold, fluorescent, observed, found, microtubules, dcasvp, cxcr, file, rna, control, expressed,

Topics {✒️}

supplemental procedures nihms631449-supplement-legends_and_supplemental_procedures figure s5 nihms631449-supplement-figure_s5 figure s2 nihms631449-supplement-figure_s2 figure s3 nihms631449-supplement-figure_s3 figure s4 nihms631449-supplement-figure_s4 mito-mcherry-24xgcn4pep + scfv-gcn4-gfp scfv-gcn4-sfgfp-2xnls-vp64 plasmids nuclease-deficient crispr/cas9 protein dcas9-suntag10x_v4-vp64-dependent gene activation mitochondrial-localized mito-mcherry-4xgcn4pep k560-suntag24x-gfp motors moved crispr-cas9-based transcription factors suntag24x-gfp-caax molecules diffused dcas9-suntag10x-vp64 induced transcription single k560-suntag24x-gfp molecules cells expressing k560-suntag24x–gfp enabling long-term imaging scfv-gcn4-gfp antibody fused dcas9-suntag10x_v4-vp64 expressing cells cas9 effector-mediated regulation pmc beta search k560rig-suntag24x-gfp molecules bound supplementary materials included sfgfp-linker-mcherry fusion protein dcas9-suntag24x_v4-gfp data showed cells expressing kif18b–suntag24x–gfp scfv-gcn4 antibody dissociates andor em-ccd camera gfp-tagged dna-binding proteins cpe-mediated translational control gcn4 antibody-peptide pair crispr rna-guided activation scfv-gcn4-sfgfp-gb1 increase α-helical propensity improve scfv-gcn4 stability nuclease-deficient mutant scfv-gcn4-gfp antibody scfv-gcn4-gfp bound dmem/10% fcs/pen/strep scfv-gcn4-gfp-vp64 optimized crispr/cas system scfv-gcn4 binding sites rna-guided gene activation spinning disk confocal suntag-dependent transcriptional activation k560rig-suntag24x-gfp revealed scfv-gcn4-gfp-nls antibody-gfp fusion bound microtubule-based motor proteins mito-mcherry-peptide array

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