
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Genome-wide profiling of mouse RNA secondary structures reveals key features of the mammalian transcriptome - PMC
Description:
The understanding of RNA structure is a key feature toward the comprehension of RNA functions and mechanisms of action. In particular, non-coding RNAs are thought to exert their functions by specific secondary structures, but an efficient annotation ...
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Keywords {🔍}
doi, rna, pubmed, google, scholar, structures, structure, pmc, cirsseq, article, free, reactivity, secondary, data, structural, analysis, rnas, average, folding, figure, regions, biol, transcripts, additional, file, coding, nucleotides, cell, mouse, lina, noncoding, binding, methods, chemical, human, mrnas, nature, region, protein, cells, supplementary, utr, method, native, ncrnas, probing, dms, mol, conformation, vivo,
Topics {✒️}
competing interests carbodiimide metho-p-toluenesulfonate cirs-seq-guided folding outperformed modification-induced reverse transcription superimposed dms/shape-reactive positions low-abundance u12 snrna cirs-seq-derived data compared superimposed dms/cmct-reactive positions cirs-seq methodology represents obtain genome-wide information superimposed shape-reactive positions perform transcriptome-wide probing cirs-seq constrained structures internal loop il2/il2′ cirs-seq data correlated cirs-seq data improves cirs-seq-derived structures integrates single-nucleotide variants embryonic stem cells protein-facilitated rna folding 1186/s13059-014-0491-2 genome-wide profiling g-rich dna flap cirs-seq data revealed stem-loop structure located pmc beta search rna-seq library preparation hela nuclear extracts vivo genome-wide profiling yeast spliceosomal u4/u6 u5 tri-snrnp particles canonical rna-seq data single-stranded rna residues de novo prediction mouse protein-coding transcripts probe protein-coding rnas protein-coding rnas revealed dms/cmct treatment induced rbp lin28a long noncoding rna long intergenic 3′ untranslated region rt stops occurred hard constraints-based methods investigate protein-masked regions low-abundance u12 snrnas sample secondary structures unmask protein-bound regions natural rt stops mouse esc rnas secondary structure inferred
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