
NCBI . NLM . NIH . GOV {
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Title[redir]:
Orthotopic and heterotopic generation of murine pancreatic cancer xenografts - PMC
Description:
For decades, mouse xenografts utilizing well-established human tumor cell lines have been the most common models used to study human cancers. More recently, βfreshβ human tumors implanted directly into immunodeficient mice have become increasingly ...
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Keywords {π}
cell, tumor, cancer, doi, mice, mouse, solution, cells, pancreatic, needle, sterile, tumors, pubmed, google, scholar, models, skin, heterotopic, xenograft, orthotopic, human, pancreas, direct, incision, implantation, site, lines, injection, cat, nodscid, media, xenografts, place, weeks, pmc, open, forceps, fragments, patient, established, growth, culture, subcutaneous, centrifuge, pipette, antibiotic, minutes, immunodeficient, figure, tab,
Topics {βοΈ}
serum-free rpmi supplemented disrupt cell clumps/cluster disrupt cell clumps/clusters disrupt cell clusters/clumps shave tumor-bearing region include long-term selection serum-free antibiotic media pmc beta search 50ml centrifuge tubes digested patient/xenograft tumor late-stage disease due nat protoc abstract recipient nod/scid mice single cell suspensions advanced pancreatic cancer vortex-agitate cell solution weigh nod/scid mice nod/scid mice continue serum-free rpmi deep subcutaneous layers/muscle gently internalize pancreas/spleen 50ml centrifuge tube collagenase iv solution pancreatic head region primary tumor samples 1% serum-free matrigel cancer cell lines collagenase iv solution26 established cell lines single tumor fragment t-cell deficient pancreatic cancer cells nat rev cancer natl cancer inst early clinical trials pathogen-free conditions shave tumor site license information pmcid severe combined immunodeficiencies balanced salt solution modelling human cancer including pancreatic cancer13 traditional cell lines cancer stem cells mouse pancreatic parenchyma palpable tumor requires nod/scid il2rgβ/β nod/scid/gamma nod/scid mouse sizeable pancreatic tumors
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