
NCBI . NLM . NIH . GOV {
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Title[redir]:
A Plug Release Mechanism for Membrane Permeation by MLKL - PMC
Description:
MLKL is crucial for necroptosis, permeabilizing membranes through its N-terminal region upon phosphorylation of its kinase-like domain by RIP3. However, the mechanism underlying membrane permeabilization is unknown. The solution structure of the ...
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Keywords {🔍}
mlkl, doi, structure, membrane, figure, google, scholar, helix, pubmed, domain, wang, cell, bundle, residues, fourhelix, fluorescence, protein, liposome, membranes, liposomes, solution, nbd, helices, necroptosis, rip, nmr, pmc, nterminal, form, leakage, observed, activity, proteins, nsa, article, binding, death, crystal, experiments, free, necrosis, bax, mechanism, data, insertion, red, nanodiscs, absence, side, phosphorylation,
Topics {✒️}
1h-15n trosy-hsqc cross-peaks 1h-15n trosy-hsqc spectrum enhanced-sensitivity triple-resonance spectroscopy ethanol-induced liver injury 1h-15n trosy-hsqc spectra 3d 1h−15n noesy-hsqc 1h-15n hsqc spectrum high-resolution nmr investigations apaf-1/caspase-9 complex initiates systemic inflamatory response 1h-15n hsqc spectra drug-induced live injury pmc beta search fluorescent probe 7-nitrobenz-2-oxa-1 13c-edited noesy-hsqc correlating c-13-alpha-beta n-terminal helical region [google scholar] mukherjee recording side-chain h-1 n-terminal sh3 domain h-1-alpha chemical-shifts n-terminal helical domain dimensional contour plots isolated n-terminal domain cross-peak intensities classified mlkl n-terminal region 1h−13c−hsqc highly α-helical protein c-terminal helix inhibits mlkl n-terminal domains c-terminal helix acts additional c-terminal residues noe cross-peak intensities transversed relaxation optimized c-terminal helix h6 supplementary materials included mlkl n-terminal domain multiple α-helices suggested n-terminal region site-directed mutagenesis gradient enhanced tnf-alpha induces broader line widths 15n resonance assignments uniformly 15n-labeled rip1-related kinase rip3 snare complex formation acquired circular dichroism rat-liver mitochondria programmed necrotic death
Questions {❓}
- Necrosis: a specific form of programmed cell death?
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