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Keywords {🔍}

collagen, doi, expression, drosophila, pubmed, google, scholar, wing, discs, fat, perlecan, body, figures, larvae, cell, vkggfp, bms, cells, igfpi, figure, vkg, vkgg, pmc, protein, shape, disc, green, article, development, proteins, wildtype, gfptrapped, larva, results, knocked, confocal, free, show, images, dev, control, subpanels, gfp, components, hemolymph, incorporation, larval, sections, biogenesis, found,

Topics {✒️}

mouse anti-heparan-sulfate 3g10 wild-type gfp-trap insertions ph4α-efb-deficient larvae supplemental experimental procedures collagenase-treated wild-type discs pmc beta search pastor-pareja jc supplementary materials included cross-species gene engineering mouse anti-gfp antibody producing gfp-tagged versions fat body-specific driver anti-mouse igg conjugated gfp-trapped strawberry notch gfp protein-trap insertion cg-gal4+gal80ts +96 hr collagen iv-dependent manner gfp-trapped alleles specifically real-time pcr system heparan-sulfate proteoglycan perlecan vkg-gfp visibly reappeared gfp-coding sequence flanked 10% sds-page gel wild-type adult wing collagen iv-deficient discs collagen-iv-dependent stability real-time pcr analysis gfp-trapped discs large functional gfp-tagged version wild-type vkg migrated double-stranded rna targeting collagen iv-deficient larvae references abrams ew copii cargo adaptor discussion functional study integrin-ecm interactions regulate study gfp-trapped proteins gfp-based rnai method anti-gfp western blots affect vkg-gfp expression de jong pj embryonic lethality reported cargo adaptor tango1 larvae lacked vkg-gfp modulate gal4-driven igfpi reduce vkg-gfp accumulation undergo extensive morphological heparan sulfate proteoglycan heparan-sulfate chains heparan sulfate chains

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• COPII-coated vesicles: flexible enough for large cargo?

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