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Keywords {🔍}

sites, dna, genome, target, offtarget, hdr, pmc, drosophila, crispr, doi, pubmed, article, targeted, google, scholar, cas, cleavage, sequence, free, site, grnas, flies, donor, efficiency, injection, vasacas, targeting, figure, dsdna, engineering, progeny, crisprcas, germline, sequences, locus, system, crisprcasmediated, grna, injected, repair, specificity, potential, efficient, gene, dshpx, expression, observed, recombination, screening, marker,

Topics {✒️}

resulting p3xp3-egfp/vasa-3xflag-nls-cas9-nls plasmid sequence encoding 3xflag-nls-cas9-nls modified p3xp3-egfp/vasa-φc31nlsattb plasmid crispr/cas-derived rna-guided endonucleases p3xp3-egfp/vasa-φc31nlsattb plasmid 2013b crispr/cas9-mediated genome engineering germ-line-specific phic31 integrases crispr/cas-mediated genome engineering optimized crispr/cas9-mediated hdr crispr/cas9-mediated genome engineering molecular reagents pbs-hsp70-cas9 [doi] [pubmed] gasiunas facilitate crispr/cas9-mediated hdr pmc beta search transgene-free genome editing error-prone ligation process vasa-cas9 flies yields crispr/cas9-mediated cleavage crispr/cas9 system promises guide rna-encoding plasmids crispr/cas9-mediated hdr rna-guided cas9 nucleases germ line-specific cas9 hs-cre virgin females direct cas9-mediated cleavage optimizing crispr/cas9 specificity pbac{sastophd-dsred} insertion crispr/cas9-mediated nhej stringent cell culture-based large-scale analyses carried homology-independent dna repair large-scale analyses conducted 2013a cas9 transcriptional activators targeted cas9-induced cleavage vasa-cas9 flies yielded streptococcus pyogenes system germline-specific cas9 expression crispr/cas systems cas9-injected wild-type assess dsdna-mediated hdr pu6-grna targeting constructs high-stringency criteria enables rna-guided complex phd-dsred-attp vector pam-adjacent perfect matches gene-specific donor templates 3xp3-dsred-frt sequence high-copy number version resulting dsh3px1conditional allele yielded dsred-positive progeny

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Analytics and Tracking {📊}

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