
NCBI . NLM . NIH . GOV {
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Title[redir]:
Precise Maps of RNA Polymerase Reveal How Promoters Direct Initiation and Pausing - PMC
Description:
Transcription regulation occurs frequently through promoter-associated pausing of RNA polymerase II (Pol II). We developed a Precision nuclear Run-On and sequencing assay (PRO-seq) to map the genome-wide distribution of transcriptionally-engaged Pol ...
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Keywords {🔍}
pausing, doi, fig, pol, genes, pmc, promoter, google, scholar, initiation, elements, pubmed, article, free, promoters, proseq, core, dna, prox, rna, sites, elongation, positions, transcription, drosophila, nucleosome, dist, polymerase, position, tss, element, downstream, hsp, average, science, resolution, focused, complex, nature, model, cell, nlm, interaction, reads, gene, consensus, lis, splicing, promoterproximal, factor,
Topics {✒️}
short nuclear-capped rnas pmc beta search pre-mrna processing factors transcriptionally-engaged pol ii increasing pro-seq density pro-seq density relative assay called pro-seq previous gro-seq studies average pro-seq profile average pro-seq density ensuring base-pair resolution high-resolution dynamic mapping pol ii-rna complexes genome-wide nuclear run distance-weighted p-values rna polymerase stalling supplementary materials included pro-seq reads map nascent rna processing rna polymerase ii actively engaged polymerase pro-seq profile transcription start site pro-seq density strong dna-nucleosome interactions rna-seq densities enriching nascent transcripts high throughput sequencing rna polymerase reveal insufficient sequencing coverage genome-wide assessment 472 “genome-wide method maps pol ii pro-seq method pro-seq enabled previous permanganate footprints genomic binding profile pol ii transcription pol ii waiting collect pol ii paused pol ii elongating pol ii �complex interaction’ model �complex interaction model shows strong associations pro-seq reads core interaction model base-pair resolution gro-seq method united states government
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