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Keywords {🔍}

coa, camkii, doi, activation, fig, caspase, extracts, cam, incubated, camkiiα, oocytes, binding, kinase, google, scholar, pubmed, acetyl, free, analyzed, data, levels, oocyte, panel, domain, egg, cambd, coasepharose, pmc, apoptosis, beads, concentrations, cell, presence, article, cytosolic, control, egta, protein, regulatory, required, mouse, death, gstpro, metabolic, activity, residues, cacam, bound, peptide, nutt,

Topics {✒️}

k292a/r296a/k300a mutant constructs mouse camkiiα k292a/r296a/k300a acyl-coa hydrolases/thioesterases occur long-chain acyl-coa hydrolase g6p-induced heat-soluble factor performed lc/ms/ms analysis anti-t286/7 phospho-camkii immunoblot pmc beta search long-term synaptic potentiation [google scholar] knudsen acyl-coa hydrolases/thioesterases 15n–1h hsqc spectra wild-type males led heat-soluble cytosolic fractions initial proteomics-based efforts heat-soluble cytosolic fraction heat-soluble cytosolic factor camkiiδ/calmodulin complex reveals requires residues k292/r296/k300 hek-293t cells transfected hek-293t cells overexpressing phospho-thr286/7 specific antibody 5 µci [γ-32p]atp 5µci [γ-32p]atp x-ray crystal structures extended experimental procedures doxorubicin-induced cell death canonical coa-dependent activation expose c-terminal residues support coa-dependent activation dhea-induced oocyte death nadph/g6p–dependent factor g6p/nadph–dependent factor inhibits dhea-induced apoptosis heat-stable factor responsible subsequent oocyte/egg apoptosis detailed experimental procedures calmodulin-dependent manner relaxation-edited difference spectrum stanford ca-egta calculator coa-488 concentration-dependent increase supplementary materials included g6p-treated extracts compared rate-limiting enzyme required coa-dependent camkii activation experimental procedures preparation coa-cam dependent activation g6p-mediated oocyte survival cam-dependent camkii activation calcium-independent camkii regulation

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