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Title[redir]:
Universal Reference RNA as a standard for microarray experiments - PMC
Description:
Obtaining reliable and reproducible two-color microarray gene expression data is critically important for understanding the biological significance of perturbations made on a cellular system. Microarray design, RNA preparation and labeling, ...
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Keywords {🔍}
microarray, rna, cell, uhrr, microarrays, spot, spots, intensity, data, human, lines, cdna, reference, doi, urr, genes, gene, coverage, background, expression, signal, google, scholar, cylabeled, analysis, batches, hybridization, mouse, line, batch, pubmed, experiments, individual, labeled, rat, umrr, urrr, brain, testis, pmc, sample, total, design, cohybridized, compared, number, stanford, min, control, intensities,
Topics {✒️}
[google scholar] dolter parametric mann-whitney test pmc beta search produce multi-gram quantities [google scholar] quackenbush low-salt wash buffer remaining small-mass organs references southern em make multi-gram batch high-salt wash buffer satisfy long-term requirements license information pmcid formaldehyde-agarose gel electrophoresis high-quality reference rna pre-labeled complementary oligonucleotides conclusions results publisher site pdf cy5/cy3 ratio results dna-binding spin cup cy5-labeled cdna derived cy5/cy3 ratio greater cy3-labeled reference cdna acknowledgments acknowledgements cy5-labeled cdna targets low-salt buffer nucleic acids res experimental cy5-labeled cdna false discovery rate high quality rna cell lines results individual cell line individual cell lines providing hybridization signal gene expression measurements nucleotide sequence attached high-quality standard kiaa0923 gene expression pmc disclaimer single cell line cell line contributes cell lines eliminates immortal cell lines rat cell lines gene expression data compare data sets agarose gel electrophoresis kiaa0923 protein gene absolute signal intensity normalizing signal output blending rna derived
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