
NCBI . NLM . NIH . GOV {
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Title[redir]:
Identification of a Selective Polymerase Enables Detection of N6-methyladenosine in RNA - PMC
Description:
N6-methyladenosine (m6A) is the most abundant mRNA modification, and has important links to human health. While recent studies have successfully identified thousands of mammalian RNA transcripts containing the modification, it is extremely difficult ...
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Keywords {🔍}
doi, google, scholar, pubmed, rna, pmc, site, article, free, incorporation, enzyme, sequence, selectivity, polymerase, methylation, tth, biol, rnas, dna, pol, mol, sites, primer, nlm, modification, figure, control, mrna, specific, position, cellular, showed, data, context, ncbi, search, detect, dai, template, efficiency, rottman, science, nucleic, res, chem, reverse, transcriptase, opposite, nonselective, presence,
Topics {✒️}
pmc beta search pmc copyright notice high-abundance cellular rnas single-molecule sequencing steady-state incorporation efficiency supplementary materials included [google scholar] 2 [google scholar] 18 [google scholar] 33 rna template/dna primer lower abundance mrnas steady-state methods33 nucleic acids res reverse transcriptase activity massively parallel sequencing plant sci lett license information pmcid successfully identified thousands united states government final edited form complementary dnas occurs incorporating thymidine triphosphate graduate research fellowship postdoctoral research fellowship competing financial interests radiolabeled dna primer consensus sequence rac general consensus sequence tth dna pol 32p-5′-labeled primer lower enzyme concentration tth pol relative context produce moderate showed strong selectivity dna-processing enzymes multiple enzymatic steps early career scientist pmc disclaimer nat chem biol data availability statements thymidine opposite unmodified naturally occurring rnas mol cell biol sequence context effects uaa sequence context human ribosomal rna painstaking digestion analysis locating modifications due 24mer template sequence total cellular rna
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