
NCBI . NLM . NIH . GOV {
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Title[redir]:
NCYM, a Cis-Antisense Gene of MYCN, Encodes a De Novo Evolved Protein That Inhibits GSK3β Resulting in the Stabilization of MYCN in Human Neuroblastomas - PMC
Description:
The rearrangement of pre-existing genes has long been thought of as the major mode of new gene generation. Recently, de novo gene birth from non-genic DNA was found to be an alternative mechanism to generate novel protein-coding genes. However, its ...
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Keywords {🔍}
ncym, mycn, human, cells, neuroblastoma, figure, expression, mice, protein, transgenic, tif, neuroblastomas, cell, gene, data, mycnncym, google, scholar, pubmed, doi, additional, gskβ, mrna, click, file, tumors, levels, double, pmc, novo, mouse, promoter, primary, article, number, activity, measured, free, genes, scale, analysis, survival, tissues, sequences, chp, assay, tumor, antibody, knockdown, control,
Topics {✒️}
quantitative real-time rt-pcr monte-carlo chi-square test gst-fused cdk1/cyclin b1 ncym-luc e-box mt ncym-luc e-box wt e-box-dependent manner chi-square independence test purified cdk1/cyclin b1 putative e-boxes exist taxonomically-restricted genes important intact upstream e-box pmc beta search putative e-box sequence kaplan–meier survival curves mycn/ncym-amplified tumors promotes ncym knockdown-mediated destabilization 129/svj×c57bl/6j mice putative e-box located mycn cis-antisense rna chemiluminescence-based detection kit full-length ncym protein protein g-sepharose beads proteasome-dependent protein degradation cleaved caspase-3–positive areas chi-squared statistic normalized pi3k/mtor-dual inhibitor pgex-4t-ncym plasmid kaplan–meier survival analysis gst-tag cleavage mediated mycn/ncym transgenic mice human protein-coding genes cox regression model polyclonal anti-ncym antibody post-terminal sequence refers n-cym1 custom shrna flag-ncym transgenic constructs de novo evolved monoclonal anti-mycn antibody rnase-free dnase mycn/ncym transgenic mouse mycn-single neuroblastoma cells mycn-amplified neuroblastoma cells mycn/ncym tg mice double transgenic mice mtor-dependent feedback inhibition functional protein-coding genes gst-ncym fusion protein mycn/ncym promoter region human mycn/ncym locus monte-carlo simulation
Questions {❓}
- Khalturin K, Hemmrich G, Fraune S, Augustin R, Bosch TC (2009) More than just orphans: are taxonomically-restricted genes important in evolution?
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