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Title[redir]:
LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1 - PMC
Description:
We recently demonstrated that the LKB1 tumour suppressor kinase, in complex with the pseudokinase STRAD and the scaffolding protein MO25, phosphorylates and activates AMP-activated protein kinase (AMPK). A total of 12 human kinases (NUAK1, NUAK2, ...
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Keywords {🔍}
lkb, kinases, mark, ampkrelated, figure, tloop, kinase, peptide, activity, doi, phosphorylated, thr, pubmed, google, scholar, protein, cells, nuak, activation, cell, phosphorylation, complex, residue, ampk, melk, ampkα, brsk, enzymes, sik, results, shown, wildtype, peptides, activated, supplementary, pmc, qik, ser, substrate, strad, expressed, phenformin, studies, inactive, hela, qsk, activate, article, mutation, catalytically,
Topics {✒️}
cyclic-amp-dependent protein kinase maldi tof–tof analysis serine/threonine-specific protein kinases polarity-inducing kinase mark/par-1 wild-type ampk-related kinases amp-activated protein kinase gst-tagged wild-type lkb1 coomassie blue-stained bands wenner-gren foundation fellowship cell-free systems demonstrated salt-inducible kinase-mediated regulation peutz–jeghers syndrome locus pmc beta search facile single-step assay putative ser/thr kinase anterior–posterior axis formation solid-phase edman sequencing mark3[t211e] mutant possessed catalytically inactive mark3[d196a] c-terminal carboxyl group cell-free systems wild-type enzyme activated drug 5-aminoimidazole-4-carboxamide riboside short t-loop peptides t-loop thr residue mutant ampk-related kinases major 32p-labelled peptide anti-diabetic drugs rosiglitazone major 32p-labelled peptides regulating ampk-related kinases akt/pkb phosphorylated nuak1 including mark/par-1 jose equivalent t-loop residue serine threonine kinase gst-ampk-related kinases high-salt diet myc-tagged mo25α/β t-loop thr residues ampk-related kinases migrated mass spectrometry facilities flag-tagged stradα/β wild-type enzyme phosphorylated ampk-related kinases significantly ser t-loop residues remaining ampk-related enzymes adenine nucleotide-independent mechanism t-loop thr phosphorylated pseudokinase ste20-related adaptor protein kinase kin1 protein kinase complement
Questions {❓}
- A specific method for activating AMP-activated protein kinase in intact cells?
- We were unable to determine the identity of the NUAK2 peptide indicated with “?
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