
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Phosphofructokinase deficiency impairs ATP generation, autophagy, and redox balance in rheumatoid arthritis T cells - PMC
Description:
T cells from RA patients are hypoglycolytic due to insufficient induction of the glycolytic activator PFKFB3, resulting in impaired autophagy and reduced ROS production.
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Keywords {🔍}
cells, pfkfb, cell, pubmed, google, scholar, doi, glucose, fig, patients, autophagy, pmc, article, free, control, levels, naive, glycolytic, expression, controls, metabolic, glycolysis, weyand, goronzy, activity, energy, production, activation, tcr, apoptotic, rheumatoid, arthritis, ros, metabolism, protein, healthy, atp, enzyme, induction, stimulation, plasmids, immunol, cellular, intracellular, apoptosis, experiments, disease, stimulated, day, shown,
Topics {✒️}
systemic lupus erythematosus anti-cd3/cd28–coated beads pentose phosphate pathway 20 µg/ml n-bret 0 µg/ml n-bret bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2 jnk–stress kinase pathway pfk-2 inhibitor n-bret inducible 6-phosphofructo-2-kinase/fructose-2 t-cell-targeted therapies pmc beta search cd3/cd28-coated beads gene-specific rna interference au-rich instability element n-bromoacetylethanolamine phosphate specific 6-phosphofructo-2-kinase inhibitor anti-cd3–coated beads anti-cd3/cd28 beads anti-cd3/cd28 microbeads pmc copyright notice including glyceraldehyde-3-phosphate dehydrogenase pma/ionomycin-induced pfkfb3 transcripts rapamycin-sensitive pathway profluorescent redox-sensitive indicators tcr-dependent ros release 6-tetramethylpiperidin-1-oxyl references almeida tcr-induced gene programs cell-free supernatants limit atp-consuming processes rate-limiting glycolytic enzyme membrane-proximal signaling events lc3-ii protein levels potent pfkfb3-inducing signal cell-internal energy sources glucose-free medium tcr cross-linking demonstrated age-inappropriate immunosenescence phenotype psuper-shpfkfb3-h2kk plasmids including tissue-specific expression diluted rt-pcr product autophagy marker lc3-ii tolerance defects originating publisher site pdf age-matched healthy individuals glucose-free conditions 6-phosphofructo-2-kinase/fructose-2 6-phosphofructo-2-kinase/fructose 2 empty-pires-gfp plasmids autophagy inhibitor 3-methyladenine cd3/cd28 beads
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