
NCBI . NLM . NIH . GOV {
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Title[redir]:
piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells - PMC
Description:
Transgenic expression of just four defined transcription factors (c-Myc, Klf4, Oct4, and Sox2) is sufficient to reprogram somatic cells to a pluripotent state1-4. The resulting induced pluripotent stem (iPS) cells resemble ES cells in their ...
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Keywords {🔍}
cells, cell, doi, fig, reprogramming, expression, supplementary, google, scholar, pubmed, lines, ips, dox, transposon, pmc, stem, factors, pcr, fibroblasts, article, transgene, embryos, pluripotent, human, mouse, embryonic, free, nature, transposition, pbtet, induction, clones, genomic, contribution, induced, pluripotency, lacz, colonies, piggybac, factor, rtpcr, gene, positive, pbips, pbs, removal, fibroblast, dpc, analysis, shown,
Topics {✒️}
5dpc rosa26-rtta-ires-gfp embryos cba-rfa-rβgpa cassette released yield pb-teto2-ires-βgeo 5′/3′tr-flanked pgk-neo cassette pb-teto2-ires-βgeo digested pb-tet-mfx induced colonies factor-removed pb-ips lines semi-quantitative rt-pcr pb-tet-mfx cocktails transgene-specific rt-pcr analysis pb-tet transposon plasmid drug-inducible transgenic system pb-pgk-neo-bpa11 pb-tet induced clones cf-150b pulse generator ires-βgeo-bpa element completely ips cell-derived pb-tet-reprogrammed clones pb-tet cell lines future cell-based therapies chromosome-specific primer set pb-reprogrammed cell lines internal transposon-specific pcrs parental rtta-mefs serve ips cell-derived component single-copy cell lines pb-ips-derived cells post-gateway cloning sites pb-tet transposon vector vector-based reverse primer 3′utr-specific reverse primers dead-family protein gene 3′utr-directed reverse primers pmc beta search transposase-stimulated pb excision generation tetracycline-regulatable promoter pb-mediated factor transposition blunt noti/hindiii fragment pb-tet-mfx transgenes pb-ips cell clumps dox-induced fibroblast pools pb-tet-mkos transposon 1mg/ml x-gal including nuclear-localized nanog cell-surface marker ssea1 pb-ips chimaeric mice pb-tet-oct4 transgene dox-independent lines passed dox-induced colonies formed ta-cloned pcr products
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