
NCBI . NLM . NIH . GOV {
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Title[redir]:
LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivo - PMC
Description:
LIN28B regulates HbF expression in erythroblasts that are cultured from umbilical cord and adult human blood. LIN28B expression manifested a more fetal-like phenotype among adult human erythroblasts.
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Keywords {🔍}
expression, doi, linb, cells, human, hbf, linboe, pubmed, google, scholar, control, blood, adult, bcla, erythroblasts, lin, figure, pmc, article, levels, hemoglobin, erythroid, cord, culture, day, free, cell, fetal, gene, mirnas, increased, copiesng, overexpression, data, rna, sponge, compared, vector, family, γglobin, protein, performed, genes, suppression, regulates, knockdown, lina, qrtpcr, phenotype, developmental,
Topics {✒️}
erythropoietin-supplemented serum-free media stage-specific trans-acting factors 20 μg/ml poly-l-lysine genome-wide association studies mscv-ires-puro-based vector hatch-marked bars represent molecular mechanisms underlying page charge payment increasing γ-globin mrna increased γ-globin expression pmc beta search cord blood-derived erythroblasts β-thalassemic cd34+ cells beta-globin gene switching high-level hbf expression anti-β-actin probe supplementary materials included 1182/blood-2012-12-472308 lin28b-mediated expression cold antibody defining qrt-pcr detection threshold methods cell culture advanced human malignancies larvae l2 stage γ-globin expression activate γ-globin lin28b-oe encoding retrovirus intramural research programs anti-human ca1 antibody standard deviation bars erythroid-related fetal genes iblot blotting system western blot analysis human subjects review human hepatocellular carcinoma lin28b expression regulates additional genes marking lin28b-kd cells compared adult erythroblasts represent adult cd34+ cells adult-derived erythroblasts embryonic stem cells transcription factor reprogramming viral vector designs license information pmcid abstract reactivation flow cytometric analysis authorship contribution lin28b-oe downregulated bcl11a supplementary figure 1a relative expression levels
Questions {❓}
- Control of haemoglobin switching by a developmental clock?
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