NCBI . NLM . NIH . GOV {
}
We began analyzing https://pmc.ncbi.nlm.nih.gov/articles/PMC3660248/, but it redirected us to https://pmc.ncbi.nlm.nih.gov/articles/PMC3660248/. The analysis below is for the second page.
Title[redir]:
Elimination of Self-Reactive T Cells in the Thymus: A Timeline for Negative Selection - PMC
Description:
Two-photon microscopy and flow cytometry reveal the timing of thymocyte death and the surprisingly close coupling between cell death and phagocytosis during negative selection in thymic slices.
Title[redir]:
Elimination of Self-Reactive T Cells in the Thymus: A Timeline for Negative Selection - PMC
Description:
Two-photon microscopy and flow cytometry reveal the timing of thymocyte death and the surprisingly close coupling between cell death and phagocytosis during negative selection in thymic slices.
Matching Content Categories {๐}
- Science
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Keywords {๐}
thymocytes, peptide, cells, negative, cell, selection, death, thymic, specific, slices, thymocyte, cdcd, pubmed, google, scholar, doi, time, data, addition, fig, imaging, arrest, migratory, min, hours, twophoton, phagocytes, caspase, pmc, apoptosis, mice, mov, tcr, article, microscopy, calcium, labeled, thymus, selecting, antigen, encounter, timelapse, video, activation, active, immunol, free, control, added, snarf,
Topics {โ๏ธ}
major histocompatibility complex-restricted cyan fluorescent protein green fluorescent protein cd4+cd8+ double positive peptideโmhc-bearing dendritic cells cd4+ sp thymocytes ca2+-free indo-1 lr ca2+-bound/ca2+-free ratio cd8+cd4โ single positive green fluorescent granulocytes ca2+-bound indo-1 lr isolated cd4+cd8+ thymocytes antigen-bearing dendritic cell extrathymic antigen-presenting cells cd4+cd8+ thymocytes exhibited cd4+cd8+tcrlo thymocytes f5 cd4+cd8+ thymocytes cd4+cd8+ f5 thymocytes double-positive thymocytes gated cd4+cd8+ thymocytes cognate peptide-mhc ligand phosphatidylserine ๏ฟฝpre-selectionโ cd4+cd8+ double-positive cells protein kinase c proapoptotic protein bim sjl-ptprca pepcb/boyj sapphire laser mai-tai cfp-expressing wt thymocytes pan-caspase inhibitor zvad-fmk phagocytosing lysm-gfp cell live/dead fixable aqua pmc beta search gfp-expressing f5 thymocytes cd4+cd8+ f5 lysm-gfp reporter mice specific pathogen-free conditions ca2+-dependent transcriptional induction lysm-gfp+ mouse overlaid antigen presenting cells antigen-presenting cells phagocytosing lysm-gfp cells phenol-free dmem indo-1 lr excitation cd4โcd8โ populations cd4+cd8+ thymocytes lethally-irradiated mice reconstituted double-labeled peptide-presenting cells lysm-gfp thymic slices
Questions {โ}
- For example, what is the sequence of events from the first encounter with a negative selecting ligand until the death of the thymocyte?
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