
NCBI . NLM . NIH . GOV {
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Title[redir]:
A photoconvertible fluorescent reporter to track chaperone-mediated autophagy - PMC
Description:
Chaperone-mediated autophagy (CMA) is a selective mechanism for the degradation of soluble proteins in lysosomes. CMA contributes to cellular quality control and is activated as part of the cellular response to different stressors. Defective CMA has ...
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Keywords {🔍}
cma, cells, kferqpscfp, doi, lysosomes, fluorescent, serum, reporter, proteins, protein, fluorescence, fig, lysosomal, cell, activity, green, pubmed, google, scholar, puncta, maintained, pmc, presence, intensity, autophagy, article, cytosolic, degradation, macroautophagy, analysis, number, expressing, bars, experiments, free, lampa, pscfp, fibroblasts, values, pathway, photoconversion, mouse, images, absence, cellular, removal, photoconvertible, conditions, cyan, stably,
Topics {✒️}
stably expressing kferq-pa-mcherry1 newly synthesized kferq-ps-cfp2 stably expressing ps-cfp2 kferq-ps-cfp2-expressing cells lentiviral kferq-ps-cfp2 construct pkferq-ps-cfp2 expression plasmid kferq-ps-cfp2-positive puncta kferq-pa-mcherry reporter make detect kferq-ps-cfp2 puncta kferq-ps-cfp2 proteins reach fluorescent kferq-ps-cfp2 puncta kferq-ps-cfp2 reporter protein pkfefq-pa-mcherry1 plasmid [pubmed] [google scholar] kferq-pa-mcherry1 construct kferq-pa-mcherry reporter revealed ppa-mcherry1-n1 vector25 kferq-ps-cfp2 construct pkferq-ps-cfp2-n1 kferq-ps-cfp2 compared kferq-ps-cfp2 colocalizes contained kferq-ps-cfp2 kferq-ps-cfp2 localized pps-cfp2-n1 vector cells expressing ps-cfp2 kferq-ps-cfp2 proteins 16h total kferq-ps-cfp2 chaperone-mediated autophagy depends kferq-pa-mcherry1 reporter pps-cfp2-n1 plasmids ph-sensitive fluorophore lysotracker kferq-ps-cfp2 reporter pmc beta search kferq-ps-cfp2 fluorescence kferq-ps-cfp2 detected high-resolution ccd camera kferq-ps-cfp2 present kferq-ps-cfp2 region mutant alpha-synuclein kferq-ps-cfp2 bound photoconverted kferq-ps-cfp2 kferq-ps-cfp2 visualized monitor chaperone-mediated autophagy serum-free complete medium kferq-ps-cfp2 expressed lamp-2a effectively eliminated bonferroni post-hoc test primary mouse fibroblasts lamp-2a-positive structures kferq-pa-mcherry1
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