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zcchc, igf, mice, mirna, mirnas, expression, figure, growth, uridylation, mature, livers, terminal, pubmed, google, scholar, doi, cell, pmc, deficiency, data, article, utr, zcchcdeficient, liver, rna, free, stem, silencing, type, embryonic, mouse, wild, cells, sequences, decreased, dayold, reporter, uridines, mirp, neonatal, sequence, uridine, libraries, sequencing, protein, gene, lin, control, survival, small,

Topics {✒️}

nih grants r01-hl079392 rna-binding zinc knuckles pmc beta search pmir-report expression vector phenol-chloroform extracted rna system-wide growth defect 18s rrna qrt-pcr zcchc11-deficient pups grew ambiguous/unrecognizable adaptor sequences n-terminal deletion mutant end-modifications observed varied homozygous zcchc11-deficient offspring zcchc11-deficient mice allowed end-modifying mature mirnas generated zcchc11-deficient mice license information pmcid mouse embryonic stem embryonic stem cells zcchc11-mediated terminal uridylation zcchc11-deficient mice revealed stem cell maintenance template-independent transfer gotaq dna polymerase cancer cell lines cell lines results physiological processes remain firefly luciferase reporter gene-trap insertion zcchc11 pre-mirna uridylation supplementary materials included full genome alignment completely eliminated repression pcmv-sport6 vector core mirna machinery β-gal insertion post-transcriptional generation represent enzymatic additions average quality score cell signaling technologies generated mice deficient β-galactosidase reporter efficient rna polyuridylation eliminate igf-1 signaling histone h3 content early blastocyst stage united states government survivors grew poorly hardy-weinberg equilibrium observe increased quantities regulating igf-1 mrna

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