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Title[redir]:
AMPK Regulates Mitotic Spindle Orientation through Phosphorylation of Myosin Regulatory Light Chain - PMC
Description:
The proper orientation of the mitotic spindle is essential for mitosis; however, how these events unfold at the molecular level is not well understood. AMP-activated protein kinase (AMPK) regulates energy homeostasis in eukaryotes, and AMPK-null ...
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Keywords {🔍}
spindle, cells, actin, ampk, fig, control, mitotic, cell, poles, doi, scholar, pubmed, google, mitosis, pmrlcser, orientation, astral, ampkdepleted, pampkthr, microtubules, sirna, cortex, lkb, kinase, mrlc, depletion, camkk, bundling, images, spindles, pole, bar, showing, levels, pmc, protein, shown, show, misoriented, hela, regulates, myosin, bundles, confocal, article, biol, misorientation, normal, function, free,
Topics {✒️}
calcium/calmodulin kinase kinase additional primary-secondary-antibody pair amp-activated/snf1 protein kinases amp-activated protein kinase pmc beta search heterotrimeric serine/threonine kinase microtubule-f-actin interaction astral microtubule-actin interactions dimethyl sulfoxide [dmso]-treated representative maximum-projection images rabbit anti-phospho-ampkthr172α ampk-null drosophila mutants myosin x-dependent manner rabbit anti-phospho-mrlcser19 integrin-mediated adhesion orients sto-609-treated cells stained ampk-depleted cells originate maximum-projection confocal images cell free lysates camkk-induced ampk activation live-cell mitotic imaging ampk sirna-treated cells mrlc sirna-treated cells ampk-depleted h1299 cells bright-field imaging ampk-depleted cells compared ampk-depleted mitotic cells ampk activates mrlc potential lkb1-ampk pathway camkk regulates ampk-mrlc jasplakinolide-treated cells compared ampk-depleted cells leads ampk-depleted hela cells supplementary materials included live-cell confocal imaging ampk depletion induced sto-609-treated hela cells actin bundling induced ampk kinase activity primary antibody overnight 100× plan-apo objective contracting skeletal muscle maximum-projection images actin-binding proteins orient myosin light chains publisher site pdf regulates energy homeostasis aberrant astral microtubules western blot showing actin directly interact
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