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Keywords {🔍}

cells, ime, doi, rtg, meiotic, cell, figure, mum, pubmed, google, scholar, development, growth, yeast, mrna, pmc, meiosis, slz, dna, imeδδ, article, free, methylation, starvation, daughter, bud, rna, medium, strains, protein, point, hours, complex, wildtype, buds, spo, data, analysis, tif, cerevisiae, levels, mis, formation, premeiotic, mother, saccharomyces, time, panel, additional, developmental,

Topics {✒️}

3x-myc-tagged ime4 protein 3x-ha-tagged mum2 protein 3x-ha-tagged slz1 protein pcup1-ime4 pcup1-mum2 pcup1-slz1 carboxy-terminal cleavage product dimensional thin-layer chromatography respective carboxy-terminal regions ime4δ/δ hyper-ph development pmc beta search clb5δ/δ clb6δ/δ double-strand break repair immunophilin-interacting protein atfip37 slz1 leucine-zipper motif ha-conjugated agarose beads ime4δ/δ mutants exhibited synthetic low-ammonium dextrose gal4 dna-binding domain ime4δ/δ flo11δ/δ ime4δ/δ flo8δ/δ nutrient-deprived yeast pass license information pmcid hyper-filamentation phenotype appeared ime2δ/δ ime4δ/δ higher molecular-weight form sex-specific splicing factor alcohol-induced filamentous growth post-transcriptional rna methylation pre-meiotic dna synthesis ha-tagged mum2 protein ha-tagged slz1 protein estradiol-inducible ndt80 construct largely completed pre-meiotic putative ime4-interaction domains publisher site pdf x-axis represents positions mrna n6-adenosine methyltransferase pcup1-ime4 pcup1-ime4 post-transcriptional rna modification pre-meiotic dna replication slz1δ/δ cells accumulated forming rtg-ph cells slz1δ/δ cells recapitulated rtg-ph developmental potential meiosis-specific protein kinase ime2-dependent meiotic initiation cell-type-specific genes enter rtg-ph development supplementary materials included form n6-methyladenosine—m6a daughter bud length = 0

Questions {❓}

• Commitment to meiosis: what determines the mode of division in budding yeast?

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