
NCBI . NLM . NIH . GOV {
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Title[redir]:
Artificial Polyploidy Improves Bacterial Single Cell Genome Recovery - PMC
Description:
Single cell genomics (SCG) is a combination of methods whose goal is to decipher the complete genomic sequence from a single cell and has been applied mostly to organisms with smaller genomes, such as bacteria and archaea. Prior single cell studies ...
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Keywords {🔍}
cells, cell, genome, doi, dna, amplification, control, single, data, pctreated, google, scholar, mda, genomic, pubmed, treatment, bias, subtilis, coverage, min, size, untreated, treated, primer, pmc, ftsz, tif, additional, lysis, sequencing, article, lbs, sorted, template, free, sets, content, qpcr, fig, population, figure, bacterial, division, plate, compared, analysis, click, file, polyploidy, study,
Topics {✒️}
dna content∼preparation+treatment+primer set+preparation pmc beta search gene-regulator binding events selective anti-staphylococcal activity complex ’supra-organisms’ consisting sequence alignment/map format pc190723-treated cells showed broad gram-positive bacteria single-cell genetic analysis publisher site pdf pre-sterilized sheath fluid illumina® gaiix™ platform supplementary materials included φ29 dna polymerase greater genomic recovery single-cell genomic sequencing de novo assemblies de novo assembly flow cytometry showed [google scholar] 43 [google scholar] license information pmcid influx™ flow cytometer instrument preparatory steps larger pc190723-treated cells bacterial protein ftsz pc190723-treated samples resulting inhibits cell division 10×φ29 reaction buffer maximum dna quantity phi29 dna polymerase greater genome coverage septal ring typically de novo sequencing 50-min pc190723-treated cells treated population showed de bruijn graphs higher genomic recovery assembled de novo targeting cell division induced artificial polyploidy create artificial polyploidy primer sets showed exhibits natural polyploidy sterilized di water single cell genomics increased dna content genomic dna content subsequent genomic analyses estimate dna content
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