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Title[redir]:
Regional deficiencies in chaperone-mediated autophagy underlie α-synuclein aggregation and neurodegeneration - PMC
Description:
In neurodegenerative diseases, it remains unclear why certain brain regions are selectively vulnerable to protein aggregation. In transgenic mice expressing human A53T α-synuclein, the brainstem and spinal cord develop the most prominent α-synuclein ...
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Keywords {🔍}
lampa, αsyn, regions, mice, cma, levels, doi, lysosomal, brain, lysosomes, degradation, protein, expression, pubmed, google, scholar, spinal, cord, activity, brainstem, figure, nontransgenic, substrate, cuervo, gapdh, human, uptake, aggregation, transgenic, kda, mouse, membrane, pmc, system, purified, disease, conditions, lamp, blot, differences, αsynuclein, article, relative, inclusions, compared, antibody, alphasynuclein, regional, previously, increased,
Topics {✒️}
wild-type α-syn modified wild-type α-syn degradation sds-page/western blot alongside wild type alpha-synuclein polyclonal anti-lamp-2a antibody wild type α-syn human a53t α-syn α-syn inclusion-forming regions lamp-1/lamp-2 double-deficient fibroblasts a53t α-syn decreased chaperone-mediated autophagy markers chaperone-mediated autophagy depends a53t α-syn mice a53t α-syn bound purified human α-synuclein detergent-insoluble α-syn van der putten a53t α-syn transgene exogenously added α-syn purified human α-syn dictate α-syn homeostasis prominent α-synuclein inclusions gene encoding alpha-synuclein minimal α-syn pathology pmc beta search o-glycan attachment sites unique lamp-2a-independent factors α-syn forms inclusions mutant alpha-synuclein linked gene encoding α-syn effectively degrade α-synuclein micro-bca protein assay alpha-synuclein locus duplication prominent α-syn aggregation human α-syn verified sigma e-degly kit developing α-syn aggregates sds-page/western blot sds/page alongside standards lysosomal enzyme β-hexosaminidase α-syn inclusion formation α-syn mouse models α-syn aggregation occurs α-syn transgene expression α-syn aggregates selectively a53t α-synuclein alpha-synuclein gene identified exogenous α-syn c-terminal cytosolic tail β-hexosaminidase activity assay
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