
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Structure of HDAC3 bound to corepressor and inositol tetraphosphate - PMC
Description:
Histone deacetylase enzymes (HDACs) are emerging cancer drug targets. They regulate gene expression by removing acetyl groups from lysine residues in histone tails resulting in chromatin condensation. The enzymatic activity of most class I HDACs ...
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Keywords {π}
hdac, doi, insp, pubmed, google, scholar, complex, pmc, smrtdad, figure, histone, deacetylase, article, free, inositol, structure, activity, dad, activation, active, site, corepressor, hdacs, residues, assembly, cells, protein, interaction, class, smrt, transcriptional, role, cell, binding, supplementary, biol, science, complexes, structural, acetate, buffer, bound, essential, mechanism, yeast, repression, domain, surface, expressed, helix,
Topics {βοΈ}
h17c/g21a/k25i/r264p/l265m/r301a n-terminal 10xhis-3xflag tag v-src-transformed rat-1 fibroblasts atp-dependent chromatin-remodeling complexes amino-terminal dad-specific motif pmc beta search dermatomyositis-specific autoantigen mi2 lc-ms/ms showed wd40-repeat protein linked site-specific variants complexed cutaneous t-cell lymphomas18 flag-tagged smrt-dad his6-tagged smrt-dad human n-cor complex h17c/g21a/k25i fo-fc density consistent size exclusion chromatography dad-specific motif forms dad-specific motif buries thyroid hormone receptor nuclear receptor corepressor methods protein expression v-src oncogene flag affinity chromatography n-cor exist bacterially-expressed smrt-dad bacterially expressed smrt-dad nuclear receptor corepressors making extensive contacts inositol phosphate multikinase myc-tagged dad n-cor corepressors histone deacetylase inhibitor pseudo helix h1 vitro reconstitution assay supplementary materials included amino-terminal portion amino terminal helix full-length hdac3 full length hdac3 salt concentrations higher conserved deacetylase-activation-domain inositol phosphate metabolism predominantly nuclear localization license information pmcid large protein complexes forms helix h1 hdac3/smrt-dad complex corepressor complexes bring physiological ionic strengths
Questions {β}
- Does Ins(1,4,5,6)P4 regulate HDACs?
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