
NCBI . NLM . NIH . GOV {
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Title[redir]:
GADD45A Protein Plays an Essential Role in Active DNA Demethylation during Terminal Osteogenic Differentiation of Adipose-derived Mesenchymal Stem Cells - PMC
Description:
Methylation and demethylation of DNA are the complementary processes of epigenetic regulation. These two types of regulation influence a diverse array of cellular activities, including the maintenance of pluripotency and self-renewal in embryonic ...
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Keywords {🔍}
osteogenic, dna, demethylation, gadda, differentiation, adscs, doi, cells, pubmed, google, scholar, expression, dlx, bglap, osterix, runx, cell, methylation, active, gene, cpg, promoters, promoter, genes, pmc, stem, article, shrna, free, hoxa, analysis, medium, methylated, fig, days, density, col, occurs, role, induction, gaddb, terminal, undifferentiated, protein, gaddg, staining, including, regions, scrambled, biol,
Topics {✒️}
ten-eleven translocation base excision repair dnmt inhibitor 5-aza-2-deoxycytidine activation-induced deaminase radical s-adenosylmethionine alkaline phosphatase quantitative pcr analysis dna methyltransferase widely ice-cold phosphate-buffered saline esc 5-methylcytosine 5-hydroxymethylcytosine gadd45g nucleotide sequences mesenchymal stem cells radical adomet-based demethylation 5-azadc-induced hypomethylation embryonic stem cell tet-produced 5hmc sterile phosphate-buffered saline dna repair p53-regulated histone-fold protein repair synthesis qrt-pcr analysis showed radical adomet domain dna damage-inducible α embryonic stem cells experimental procedures isolation protein a-sepharose coated precoated protein a-sepharose tet proteins catalyze pmc beta search cmv-egfp expression cassette rabbit anti-gadd45a antibodies adipose tissue packaging vectors pcmv-dr8 adipose fragments qrt-pcr analysis stem cell properties pmc copyright notice stem cell differentiation embryonic genome demethylation gene-specific demethylation occurs detectable aid expression osteogene-specific gene promoters supplementary materials included adult stem cells chip-qpcr analysis demonstrated gene-specific dna methylation dnmt inhibition 5-azadc-pretreated cells
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