
NCBI . NLM . NIH . GOV {
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Title[redir]:
Metformin suppresses pregnane X receptor (PXR)-regulated transactivation of CYP3A4 gene - PMC
Description:
Metformin is widely used in the treatment of type-2 diabetes. The pleotropic effects of metformin on glucose and lipid metabolism have been proposed to be mediated by the activation of AMP-activated protein kinase (AMPK) and the subsequent ...
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Keywords {🔍}
metformin, doi, cypa, expression, pxr, cells, mrna, effect, google, scholar, fig, pubmed, shp, human, gene, ampk, receptor, hepatocytes, construct, protein, data, primary, activation, cell, nuclear, activity, significant, significantly, luciferase, reporter, ligand, lst, compared, experiments, statistically, pmc, mice, levels, suppresses, transactivation, regulation, rifampicin, receptors, normalized, wildtype, assay, mouse, article, effects, induction,
Topics {✒️}
semi-quantitative real-time rt-pcr double mutant pm-gal4-pxrmut antibiotic-free dmem supplemented p3a4-luc reporter construct amp-activated protein kinase n-dimethylimidodicarbonimidic diamide hydrochloride amp/atp ratio leading important drug/xenobiotic-metabolizing enzymes crucial drug/xenobiotic enzyme prototype ligand-induced transactivations pmc beta search myc-tagged expression constructs constitutively active pxrmut main drug-metabolizing enzyme drug-metabolizing cytochromes p450 c57bl/6 pxr wild-type mz-hep1 cell lines long-term human hepatocytes pxr wild-type construct wild-type pxr construct untreated mz-hep1 cells key drug-metabolizing enzyme pxr-mediated gene expression ligand-activated nuclear receptors intestine-derived cell lines renilla luciferase activity dominant negative mutant nuclear receptor-mediated expression p3a4-luc construct serum-free medium xenobiotic-induced transcriptional regulation pgal4 fusion construct pxr wild-type mice constitutively active form vp16-pxr lbd constructs transient transfection assays ligand binding pocket pxr-mediated cyp3a4 transactivation lh31 hepatocyte preparations gene reporter assays double pxr mutant small heterodimer partner firefly luciferase activities transfected mz-hep1 cells mz-hep1 cells transfected luciferase activity normalized protein kinase activators rifampicin-stimulated cyp3a4 expression ligand binding domain dominant negative form
Questions {❓}
- Metformin--the gold standard in type 2 diabetes: what does the evidence tell us?
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