
NCBI . NLM . NIH . GOV {
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Title[redir]:
Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells - PMC
Description:
Cooperatively assembled signalling complexes, nucleated by adaptor proteins, integrate information from surface receptors to determine cellular outcomes. In T and mast cells, antigen receptor signalling is nucleated by three adaptors: SLP-76, Gads ...
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Keywords {🔍}
slp, phosphorylation, cells, google, scholar, pubmed, plcγ, cell, doi, activation, itk, figure, tyrosine, pmc, article, mutation, sites, free, required, mast, phosphorylated, calcium, experiments, stimulation, wildtype, signalling, tcrinduced, proteins, kinase, domain, activity, receptor, signaling, biol, koretzky, nterminal, results, protein, reduced, bogin, antigen, complex, luciferase, adaptor, vav, flux, zap, tcr, interaction, supplementary,
Topics {✒️}
involve calcium–calcineurin-mediated dephosphorylation pe-cy5-conjugated anti-cd69 gst-plc-γ1sh2−sh2−sh3 rabbit monoclonal anti-phospho-p38 activates phospholipase c-gamma1 polyclonal anti-phospho-p44/42 mapk phosphospecific anti-plc-γ1 py783 wild-type bone marrow flag-tagged wild-type slp-76 pmigr1-based retroviral plasmids n-terminal sam domain fcεri-induced plc-γ1 activation c-terminal sh2 domain pmc beta search calcium–phosphate-mediated cotransfection ires-gfp-marked retroviral vector high-affinity ige receptor tcr-induced tyrosine-phosphorylated proteins tcr-induced calcium levels calcium-dependent effector functions n-terminal acidic domain thapsigargin-induced calcium flux phospholipase c-γ isoforms anti-mouse cd3ε-biotin monoclonal anti-flag epitope jurkat-derived cell lines flag-tagged wild-type central proline-rich domain antigen receptor-induced phosphorylation tcr-stimulated wild-type cells tcr-induced calcium flux tec-family tyrosine kinase modulate t-cell responsiveness fcεri-induced calcium flux n-terminal phosphorylation sites anti-human cd3-apc tcr-induced luciferase activity β-hexosaminidase activity released b-cell adaptor analogous cell receptor-induced activation nfat-luciferase reporter plasmid ras-dependent activation marker plc-γ1-dependent responses cell-mediated immune responses tcr-induced tyrosine phosphorylation calcium-free indo1 retrovirally reconstituted wild-type flag-tagged human slp-76 tcr-induced surface expression phospholipase c-γ1 depends
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